EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   2.7.10.2 | -999 |
- |
more |
- |
661180 |
| 2.7.10.2 | -999 |
- |
more |
the overall activity kcat of the catalytic domain Src, Src-CD, including the C-terminal tail for two exogenous substrates, the Src substrate peptide AEEEIYGEFEAKKKK and denatured enolase is about 10 times higher than that of wild-type Src. The kcat values for phosphorylation of the Src substrate peptide are similar for the unphosphorylated and monophosphorylated Src-CD, 50 min-1 |
490075 |
| 2.7.10.2 | 0.01 |
- |
poly(Glu4-Tyr) |
pH 8.0, 30°C, recombinant Csk residue-339-deletion mutant |
662138 |
| 2.7.10.2 | 0.072 |
- |
EEEEY |
pH 8.0, 30°C, recombinant mutant H384Y/Y416F |
672104 |
| 2.7.10.2 | 0.1 |
- |
EEEEY |
pH 8.0, 30°C, recombinant mutant H384A |
672104 |
| 2.7.10.2 | 0.1 |
- |
poly(Glu4-Tyr) |
pH 8.0, 30°C, recombinant mutant E338A Csk |
662138 |
| 2.7.10.2 | 0.11 |
- |
[kdSrc kinase]-L-tyrosine |
pH 8.0, 30°C, recombinant mutant R281A/R283A |
663196 |
| 2.7.10.2 | 0.13 |
- |
[kdSrc kinase]-L-tyrosine |
pH 8.0, 30°C, recombinant mutant S280A/R281A/R283A/F381A |
663196 |
| 2.7.10.2 | 0.15 |
- |
EEEEY |
pH 8.0, 30°C, recombinant mutant H384Q/Y416F |
672104 |
| 2.7.10.2 | 0.16 |
- |
ATP |
pH 8.0, 30°C, recombinant mutant H384Y/Y416F |
672104 |
