Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is activated by phosphorylation, and also performs autophosphorylation | Homo sapiens |
Application | Comment | Organism |
---|---|---|
drug development | tyrosine kinase Src is a key enzyme in mammalian signal transduction and an important target for anticancer drug discovery | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of active Src in Escherichia coli strain BL21(DE3) is difficult and ineffective due to toxicity caused by Src kinase activity, effective expression of inactive mutants, e.g. of K295M, is possible, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H384A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Src | Homo sapiens |
H384Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Src | Homo sapiens |
H384Q/Y416F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Src | Homo sapiens |
H384Y | site-directed mutagenesis, the mutant shows reduced activity and no autophosphorylation compared to the wild-type Src | Homo sapiens |
H384Y/Y416F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Src | Homo sapiens |
H384Y/Y527F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Src | Homo sapiens |
K295M | site-directed mutagenesis, inactive Src mutant | Homo sapiens |
N391D | site-directed mutagenesis, the mutant shows highly reduced enzyme activity and can be well expressed and purified using the Escherichia coli expression system | Homo sapiens |
R388A/A390R | site-directed mutagenesis, mutations in the catalytic loop, the mutant shows slightly reduced enzyme activity and cannot be well expressed and purified using the Escherichia coli expression system | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
0.075 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.253 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y/Y527F | Homo sapiens | |
0.309 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y | Homo sapiens | |
0.327 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Q | Homo sapiens | |
0.335 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y/Y416F | Homo sapiens | |
0.34 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384A | Homo sapiens | |
0.345 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Q/Y416F | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | tyrosine kinase Src is a key enzyme in mammalian signal transduction and an important target for anticancer drug discovery | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is activated by phosphorylation, and also performs autophosphorylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant inactive Src mutants from Escherichia coli strain BL21(DE3) by affinity chromatography, Src mutants with kinase activity above a certain threshold cannot be purified from a bacterial expression system, while a variety of mutants with a kinase activity below this threshold can be expressed and purified, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EEEEY | - |
Homo sapiens | ADP + EEEE(phospho)Y | - |
? | |
additional information | tyrosine kinase Src is a key enzyme in mammalian signal transduction and an important target for anticancer drug discovery | Homo sapiens | ? | - |
? | |
additional information | the enzyme is activated by phosphorylation, and also performs autophosphorylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Src | - |
Homo sapiens |
Src protein tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384Y/Y416F | Homo sapiens | |
0.1 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384A | Homo sapiens | |
0.15 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384Q/Y416F | Homo sapiens | |
0.16 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y/Y416F | Homo sapiens | |
0.21 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384A | Homo sapiens | |
0.22 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384Y | Homo sapiens | |
0.4 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Q/Y416F | Homo sapiens | |
0.43 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384Y/Y527F | Homo sapiens | |
0.53 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y | Homo sapiens | |
0.8 | - |
EEEEY | pH 8.0, 30°C, recombinant mutant H384Q | Homo sapiens | |
0.98 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Y/Y527F | Homo sapiens | |
1.93 | - |
ATP | pH 8.0, 30°C, recombinant mutant H384Q | Homo sapiens | |
2.27 | - |
EEEEY | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
3.13 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
3.52 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |