Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant Csk in Escherichia coli strain DH5alpha | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D276A | site-directed mutagenesis, reduced activity with substrates poly(Glu4-Tyr) and [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
E300A | site-directed mutagenesis, reduced activity with substrates poly(Glu4-Tyr) and [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
F382A | site-directed mutagenesis, reduced activity with substrate poly(Glu4-Tyr), and highly redcuced activity with substrate [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
R281A/R283A | site-directed mutagenesis, about 90% reduced activity with substrate [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
S273A | site-directed mutagenesis, reduced activity with substrate [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
S280A | site-directed mutagenesis, reduced activity with substrates poly(Glu4-Tyr) and [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
S280A/R281A/R283A/F381A | site-directed mutagenesis, about 90% reduced activity with substrate [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
S284A | site-directed mutagenesis, reduced activity with substrates poly(Glu4-Tyr) and [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
S381A | site-directed mutagenesis, reduced activity with substrates poly(Glu4-Tyr) and [kdSrc kinase]-L-tyrosine compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of the Src phosphorylation by peptides mimicking the substrate binding site, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.0139 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant R281A/R283A | Homo sapiens | |
0.0229 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant S280A/R281A/R283A/F381A | Homo sapiens | |
0.095 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
0.274 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant mutant R281A/R283A | Homo sapiens | |
0.322 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant mutant S280A/R281A/R283A/F381A | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate | substrate binding site involving residues R279, R281, and R283, more distant residues S280, F382, S273, and D276 are also important for activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
ATP + [kdSrc kinase]-L-tyrosine | i.e. kinase-defective chicken Src mutant K295M | Homo sapiens | ADP + [kdSrc kinase]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-terminal Src kinase | - |
Homo sapiens |
Csk | - |
Homo sapiens |
Protein tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant R281A/R283A | Homo sapiens | |
0.13 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant S280A/R281A/R283A/F381A | Homo sapiens | |
0.9 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
1.49 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
1.85 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant mutant S280A/R281A/R283A/F381A | Homo sapiens | |
2.07 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant mutant R281A/R283A | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |