EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
4.3.3.7 | 20 |
95 |
purified recombinnat wild-type enzyme |
713953 |
4.3.3.7 | 46.5 |
- |
melting temperature in the absence of substrates |
702445 |
4.3.3.7 | 50 |
60 |
thermostability is significantly enhanced in the presence of the substrate pyruvate |
704696 |
4.3.3.7 | 59.5 |
- |
melting temperature in the absence of substrates |
702445 |
4.3.3.7 | 59.8 |
- |
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart |
702448 |
4.3.3.7 | 60 |
- |
1 min, 50% loss of activity, 5 min, complete inactivation |
33902 |
4.3.3.7 | 60 |
- |
inactivation energy: 67300 cal/mol, change in free energy, DELTAF: 22500 cal/mol, change in enthalpy, DELTAH: 66700 cal/mol, change in entropy DELTAS: 133 cal/degree/mol |
33908 |
4.3.3.7 | 61.3 |
- |
is thermally stabilised by the first substrate, pyruvate |
702448 |
4.3.3.7 | 65 |
- |
the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C |
715195 |
4.3.3.7 | 74 |
79 |
inactivation energy: 117000 cal/mol, change in free energy, DELTAF: 23400 cal/mol, change in enthalpy, DELTAH: 113500 cal/mol, change in entropy DELTAS: 259 cal/degre e/mol |
33905 |