EC Number   |
Source Tissue |
Reference |
|---|
    3.5.1.1 | cell culture |
optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h |
-, 719200 |
| 3.5.1.1 | cell culture |
solid state, production evaluation and optimization of culture conditions using factorial designs |
-, 657205 |
| 3.5.1.1 | cell suspension culture |
optimization of production of L-asparaginase by strain MK-VL_113. Optimal production at pH 7.0 and 30°C in a 72 h old culture in modified ISP-5 medium containing yeast extract, tryptone, or L-asparagine at 1-1.5% w/v as nitrogen source and 2% glycerol as carbon source, overview |
-, 721060 |
| 3.5.1.1 | commercial preparation |
- |
667907, 668077, 669496, 753859 |
| 3.5.1.1 | culture condition |
carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production |
686239 |
| 3.5.1.1 | culture condition |
high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source |
686239 |
| 3.5.1.1 | culture condition |
L-asparaginase-I is constitutive |
686239 |
| 3.5.1.1 | culture condition |
L-asparaginase-II is secreted in response to N starvation |
686239 |
| 3.5.1.1 | culture condition |
sabourand dextrose broth yields maximum growth and maximum L-asparaginase production |
686239 |
| 3.5.1.1 | culture condition:D-glucose-grown cell |
study of the effect of various carbon sources on pH of the broth and L-asparaginase production |
-, 713442 |
