EC Number   |
Reference |
|---|
   3.4.24.B4 | a chromatographic method is used to refold the Histidine-tagged (His-tagged) catalytic domain of MMP-13. After the optimization of gradient elution modes and chromatographic conditions, the recovery yield reaches 83% with a relative activity of 92%. Addition of small molecule inhibitors to prevent slight autoproteolytic degradation does not affect the secondary structure of the protein |
754368 |
| 3.4.24.B4 | in 50 mM MES, pH 6.5, 500 mM NaCl, 10 mM CaCl2, 1.0 mM ZnCl2, and decreasing concentrations of urea (4-0 M) |
720193 |
| 3.4.24.B4 | recombinant enzyme form inclusion bodies after expression in Escherichia coli |
652045 |
