Literature summary for 3.4.24.B4 extracted from

Zhang, R.; Zhang, L.; Li, C.; Chen, B.; Li, Q.; Fang, X.; Shen, Y.
Refolding of recombinant histidine-tagged catalytic domain of MMP-13 from escherichia coli with ion-exchange chromatography for higher bioactivity (2015), J. Liq. Chromatogr. Relat. Technol., 38, 561-568 .

No PubMed abstract available

Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification and refolding of recombinant His-tagged catalytic domain	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
a chromatographic method is used to refold the Histidine-tagged (His-tagged) catalytic domain of MMP-13. After the optimization of gradient elution modes and chromatographic conditions, the recovery yield reaches 83% with a relative activity of 92%. Addition of small molecule inhibitors to prevent slight autoproteolytic degradation does not affect the secondary structure of the protein	Homo sapiens

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Release 2023.1 (January 2023)