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Literature summary for 3.4.24.B4 extracted from

  • Deng, S.J.; Bickett, D.M.; Mitchell, J.L.; Lambert, M.H.; Blackburn, R.K.; Carter, H.L., 3rd; Neugebauer, J.; Pahel, G.; Weiner, M.P.; Moss, M.L.
    Substrate specificity of human collagenase 3 assessed using a phage-displayed peptide library (2000), J. Biol. Chem., 275, 31422-31427.
    View publication on PubMed
Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
matrix metalloprotease inhibitor GW9471 broad spectrum Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded enzyme form Escherichia coli Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme form inclusion bodies after expression in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl-GPLGMRGL-NH2 + H2O
-
 Homo sapiens ?
-
 ?
aggrecan + H2O
-
 Homo sapiens ?
-
 ?
alpha2-chain of type IV collagen + H2O cleavage sites at amino acid residues 881 and 1000 in the triple-helical region Homo sapiens ?
-
 ?
alpha4-chain of type IV collagen + H2O cleavage site at amino acid residue 86 in the triple-helical region Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
protein + H2O substrate specificity and cleavage site determination via phage display approach Homo sapiens peptides
-
 ?
Transforming growth factor + H2O cleavage site at amino acid residue 150 in the latency-associated peptide Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
M10.013 Merops-ID Homo sapiens
matrix metalloproteinase-13
-
 Homo sapiens
MMP-13
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000005
-
 matrix metalloprotease inhibitor GW9471 37°C, recombinant enzyme Homo sapiens