EC Number   |
Application |
Reference |
|---|
    3.1.8.1 | agriculture |
agricultural soils are often co-contaminated with different types of pesticides. For example, organophosphates and pyrethroids usually are applied together for pest control. So far, microorganisms with both OP- and pyrethroid-degrading activity have not been isolated from natural environments. Alternatively, construction of engineered microbes with broad-spectrum pesticide-degrading activity may be a promising strategy for bioremediation of mixed pesticides-contaminated soil. To construct multifunctional pesticide degrading microorganisms, the co-expression of multiple degrading enzymes in a host strain may be a feasible approach. Both MPH and PytH are coexpressed in a soil bacterium Pseudomonas putida KT2440, resulting in an engineered strain with the capability to simultaneously degrade OPs and pyrethroids. Six pesticides (methyl parathion, fenitrothion, chlorpyrifos, permethrin, fenopropathrin, cypermethrin) can be effectively degraded by the engineered Pseudomonas putida KT2440. Therefore, the engineered Pseudomonas putida KT2440 could potentially be applied for in situ bioremediation of soil co-contaminated with organophosphates and pyrethroids |
749988 |
| 3.1.8.1 | analysis |
description of an easy and non-toxic method for large scale phenotyping and activity quantitation of arylesterase |
35215 |
| 3.1.8.1 | analysis |
development of a polymerized crystalline colloidal array photonic crystal sensing material which reversibly senses the organophosphate compound methyl paraoxon at micromolar concentrations in aqueous solutions using the enzyme, mechanism, overview |
677515 |
| 3.1.8.1 | analysis |
PON1 has the potential to be used as a catalytic bioscavenger of nerve agents. Insect production of PON1 may provide a source for both in vitro enzymatic and crystallographic studies and in vivo stability and anti-nerve agent efficacy testing |
708095 |
| 3.1.8.1 | analysis |
PON1 status can be used as a potential biomarker for insecticides metabolites in urine, e.g. dimethyl phosphate and diethyl phosphate, and of susceptibility to organophosphate toxicity, overview |
664630 |
| 3.1.8.1 | biotechnology |
chemo-enzymatic procedure for chiral synthesis of soman analogues, use of mutants to reverse stereoselectivity |
646541 |
| 3.1.8.1 | biotechnology |
future biotechnological developments of PTE as a detoxifying enzyme |
709292 |
| 3.1.8.1 | degradation |
a series of substituted phenoxyalkyl pyridinium oximes enhance the degradation of surrogates of sarin (i.e. nitrophenyl isopropyl methylphosphonate, NIMP) and VX (i.e. nitrophenyl ethyl methylphosphonate, NEMP). Neither NIMP nor NEMP is hydrolyzed effectively by paraoxonase PON1 if one of these oximes is absent. In the presence of eight novel oximes, PON1-mediated degradation of both surrogates occurs |
731000 |
| 3.1.8.1 | degradation |
activity and stability of organophosphorus hydrolase are enhanced by interactions between the hydrophobic poly(propylene oxide) block of amphiphilic Pluronics and the enzyme. The strategy provides an efficient route to new formulations for decontaminating organophosphate neurotoxins |
729582 |
| 3.1.8.1 | degradation |
the enzyme is used for the detoxification of organophosphate pesticides and realted chemical warfare agents such as VX and sarin |
666526 |
