EC Number   |
Application |
Reference |
|---|
   2.4.1.65 | molecular biology |
the stable system using the expression vector pIB/Vf-His-TOPO constitutes an advance for the large scale expression of glycosyltransferases and possibly other glycoproteins in insect cells |
662484 |
| 2.4.1.65 | synthesis |
alpha1-3/4-fucosyltransferase is expressed in Escherichia coli at a level of 30 mg/l culture and used as a diverse catalyst in a one-pot multienzyme system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, x, O-sulfated Lewis x, and sialyl Lewis x as well as human milk fucosides such as 3-fucosyllactose, lacto-N-fucopentaose III, lacto-N-difuco-hexaose II and III. While difucosylation of tetrasaccharides is readily achieved using an excess amount of donor, synthesis of lacto-N-fucopentaose III is achieved by alpha1-3/4-fucosyltransferase-catalyzed selective fucosylation of the N-acetyllactoamine component in lacto-N-neotetraose |
756431 |
| 2.4.1.65 | synthesis |
by constructing yeast cells that display human FUT6 on the cell wall by fusion of FUT6 with the yeast cell wall proteins Pir1 and Pir2, the fucosylated oligosaccharides can be easily synthesized by the incubation of yeast cells with an appropriate donor and acceptor. It will thus be possible to prepare a large amount of immobilized FUT6 fused with Pir proteins in an inexpensive medium lacking the serum that is required for mammalian cell cultivation |
661803 |
| 2.4.1.65 | synthesis |
the enzyme possesses a broad tolerance toward nonnatural type I acceptor substrate analogs and therefore represents a valuable tool for the chemoenzymatic synthesis of Lewis A, sialyl Lewis A as well as mimetics thereof |
680029 |
| 2.4.1.65 | synthesis |
the soluble form of fucosyltransferase III secreted by stably transfected cells may be used for in vitro synthesis of the Lewis 1 determinant on carbohydrates and glycoproteins |
637648 |
