Application | Comment | Organism |
---|---|---|
synthesis | alpha1-3/4-fucosyltransferase is expressed in Escherichia coli at a level of 30 mg/l culture and used as a diverse catalyst in a one-pot multienzyme system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, x, O-sulfated Lewis x, and sialyl Lewis x as well as human milk fucosides such as 3-fucosyllactose, lacto-N-fucopentaose III, lacto-N-difuco-hexaose II and III. While difucosylation of tetrasaccharides is readily achieved using an excess amount of donor, synthesis of lacto-N-fucopentaose III is achieved by alpha1-3/4-fucosyltransferase-catalyzed selective fucosylation of the N-acetyllactoamine component in lacto-N-neotetraose | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + lacto-N-neotetraose | - |
Helicobacter pylori | GDP + ? | - |
? |