Application | Comment | Organism |
---|---|---|
synthesis | the enzyme possesses a broad tolerance toward nonnatural type I acceptor substrate analogs and therefore represents a valuable tool for the chemoenzymatic synthesis of Lewis A, sialyl Lewis A as well as mimetics thereof | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | 10 mM, 90% inhibition | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00573 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
0.111 | - |
Siaalpha-2,3Galbeta-1,3GlcNAc-R | 37°C, pH 6.5 | Helicobacter pylori | |
0.114 | - |
Galbeta-1,3GlcNAc-R | 37°C, pH 6.5 | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50502 | - |
x * 50502, SDS-PAGE | Helicobacter pylori |
64500 | - |
x * 64500, SDS-PAGE | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | Q6ST35 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Helicobacter pylori |
Storage Stability | Organism |
---|---|
-70°C, recombinant enzyme is stable for several months in cacodylate buffer containing | Helicobacter pylori |
10% glycerol. | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + Gal-beta-(1->3)-GlcNAc-beta-O-(CH2)8COOCH3 | - |
Helicobacter pylori | GDP + Gal-beta-(1->3)-[Fuc-alpha-(1->4)]-GlcNAc-beta-O-(CH2)8COOCH3 | - |
? | |
GDP-beta-L-fucose + Galbeta-1,3GlcNAc-R | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3 | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Siaalpha-2,3Galbeta-1,3GlcNAc-R | - |
Helicobacter pylori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64500, SDS-PAGE | Helicobacter pylori |
? | x * 50502, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4 fucosyltransferase | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | - |
Helicobacter pylori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: 70% of maximal activity, 50°C: about 60% of maximal activity | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.13 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
3.947 | - |
GDP-beta-L-fucose | 37°C, pH 6.5 | Helicobacter pylori | |
8.1 | - |
Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori | |
26.12 | - |
Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori | |
34.33 | - |
NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3 | 37°C, pH 6.5 | Helicobacter pylori |