EC Number   |
Organic Solvent |
Reference |
|---|
    1.1.1.47 | 1-hexanol |
the enzyme exhibits an extreme tolerance towards 1-hexanol with 6%loss of activity at 10% (v/v) |
-, 739842 |
| 1.1.1.47 | 1-phenylethanol |
mutant enzymes Q252L/E170K/S100P and Q252L/E170K/K166R display a 2.9fold and 2.0fold improvement in tolerance against 1-phenylethanol, respectively, compared with mutant enzyme Q252L/E170K. The half-life (t1/2) of mutant enzyme Q252L/E170K/S100P/K166R in the presence of 10% (v/v) 1-phenylethanol is prolonged from 70.4 min to 319 min, representing a 4.5fold increase compared with mutant enzyme Q252L/E170K. The chemical tolerance of mutant enzyme Q252L/E170K/S100P/K166R/V72I and mutant enzyme Q252L/E170K/S100P/K166R/K137R against 10% (v/v) 1-phenylethanol is increased by 5.6fold and 6.7fold, respectively, compared with mutant enzyme Q252L/E170K |
-, 760889 |
| 1.1.1.47 | 2-hexanol |
the enzyme exhibits an extreme tolerance towards 2-hexanol with 7%loss of activity at 50% (v/v) |
-, 739842 |
| 1.1.1.47 | Acetone |
30%: nearly 100% activity after 1h at 25°C |
-, 711501 |
| 1.1.1.47 | Acetone |
50% v/v, room temperature, 6 h, stable |
639106 |
| 1.1.1.47 | Acetone |
50%, 24 h, room temperature, no loss of activity |
639110 |
| 1.1.1.47 | Acetone |
enzyme exhibited high tolerance to 30% acetone |
711501 |
| 1.1.1.47 | acetophenone |
mutant enzyme Q252L/E170K retains 8% of initial activity when incubated for 12 h with 10% acetophenone |
-, 760889 |
| 1.1.1.47 | cyclohexane |
enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide in cyclohexane with n-butanol as cosurfactant and retaines activity and catalytic properties in the organic medium |
654400 |
| 1.1.1.47 | DMSO |
60%: nearly 100% activity after 1h at 25°C |
711501 |
