General Stability | Organism |
---|---|
0.05% SDS, no loss of activity, 0.1%, slow decrease | Saccharolobus solfataricus |
2 M urea, 24 h, room temperature, no loss of activity, slow decrease at 4 M | Saccharolobus solfataricus |
4 M guanidinium chloride, rapid inactivation | Saccharolobus solfataricus |
H4-furan, rapid inactivation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, room temperature, pH 9.0, rapid inactivation | Saccharolobus solfataricus | |
5,5'-dithiobis(2-nitrobenzoic acid) | 1 mM, room temperature, pH 9.0, 50% inactivation | Saccharolobus solfataricus | |
EDTA | 50 mM, 5% activity left | Saccharolobus solfataricus | |
N-ethylmaleimide | 3 mM, room temperature, pH 9.0, 50% inactivation | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADP+ | pH 9.0, 70°C | Saccharolobus solfataricus | |
0.44 | - |
beta-D-glucose | pH 9.0, 70°C, coenzyme NADP+ | Saccharolobus solfataricus | |
1.2 | - |
NAD+ | pH 9.0, 70°C | Saccharolobus solfataricus | |
2.2 | - |
beta-D-glucose | pH 9.0, 70°C, coenzyme NADP+ | Saccharolobus solfataricus | |
8 | - |
beta-D-glucose | pH 9.0, 70°C, coenzyme NAD+ | Saccharolobus solfataricus | |
22 | - |
beta-D-galactose | pH 9.0, 70°C, coenzyme NADP+ | Saccharolobus solfataricus | |
68 | - |
beta-D-xylose | pH 9.0, 70°C, coenzyme NAD+ | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal activity at 20 mM | Saccharolobus solfataricus | |
Mg2+ | maximal activity at 20 mM | Saccharolobus solfataricus | |
Mn2+ | maximal activity at 20 mM | Saccharolobus solfataricus | |
Zn2+ | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Saccharolobus solfataricus |
124000 | - |
gel filtration | Saccharolobus solfataricus |
130000 | - |
sedimentation equilibrium | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 50%, 24 h, room temperature, no loss of activity | Saccharolobus solfataricus |
Ethanol | 100%, slow decrease | Saccharolobus solfataricus |
Methanol | 50%, 24 h, room temperature, no loss of activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, SP-Sephadex, Phenyl-Sepharose, Affi-Gel Blue | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
437 | - |
- |
Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
4°C, 20 mM MgCl2, 20% ethylene glycol, several months, stable | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-2-deoxy-D-glucose + NAD+ | 26% of the activity with D-glucose | Saccharolobus solfataricus | 2-amino-2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-amino-2-deoxy-D-glucose + NADP+ | 5% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | 2-amino-2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
2-deoxy-D-glucose + NAD+ | 12% of the activity with glucose | Saccharolobus solfataricus | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NADP+ | 25% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
6-deoxy-D-glucose + NAD+ | 66% of the activity with D-glucose | Saccharolobus solfataricus | 6-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
6-deoxy-D-glucose + NADP+ | 9% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | ? + NADPH | - |
? | |
beta-D-glucose + NAD+ | 9% of the activity with NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NADP+ | - |
Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-xylose + NAD+ | 26% activity compared to D-glucose | Saccharolobus solfataricus | D-xylono-1,5-lactone + NADH + H+ | - |
r | |
D-allose + NAD+ | 8% of the activity with D-glucose | Saccharolobus solfataricus | D-allono-1,5-lactone + NADH | - |
? | |
D-allose + NADP+ | 13% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-allono-1,5-lactone + NADPH | - |
? | |
D-altrose + NAD+ | 5% of the activity with D-glucose | Saccharolobus solfataricus | D-altrono-1,5-lactone + NADH | - |
? | |
D-altrose + NADP+ | 12% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-altrono-1,5-lactone + NADPH | - |
? | |
D-galactose + NAD+ | 15% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-galactono-1,5-lactone + NADH | - |
? | |
D-gulose + NAD+ | 8% of the activity with D-glucose | Saccharolobus solfataricus | D-gulono-1,5-lactone + NADH | - |
? | |
D-gulose + NADP+ | 12% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-gulono-1,5-lactone + NADPH | - |
? | |
D-idose + NAD+ | 65% of the activity with D-glucose | Saccharolobus solfataricus | D-idono-1,5-lactone + NADH | - |
? | |
D-idose + NADP+ | 12% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-idono-1,5-lactone + NADPH | - |
? | |
D-mannose + NADP+ | no activity | Saccharolobus solfataricus | D-mannono-1,5-lactone + NADPH | - |
? | |
D-ribose + NADP+ | 4% of the activity with D-glucose and NADP+ | Saccharolobus solfataricus | D-ribono-1,5-lactone + NADPH | - |
? | |
D-xylose + NAD+ | 28% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NADP+ | 28% of the activity with D-glucose and NAD+ | Saccharolobus solfataricus | D-xylono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
at pH 8.0 | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 77 | 37°C: 20% of maximal activity, 77°C: optimum | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |