EC Number |
Metals/Ions |
Reference |
---|
4.2.1.2 | Ca2+ |
has a small stimulatory effect |
715093 |
4.2.1.2 | Fe |
enzyme contains an oxygen-sensitive [4Fe-4S] cluster |
679910 |
4.2.1.2 | Fe2+ |
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol |
749072 |
4.2.1.2 | Fe2+ |
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol |
746828 |
4.2.1.2 | Iron |
fumarase A contains a catalytically active [4Fe-4S]cluster |
33770 |
4.2.1.2 | Iron |
in cells grown without aeration the Fe-S-independent enzyme form occupies over 80% of the overall fumarase. In aerobically grown cells the Fe-S-dependent fumarase occupies 80% of the overall activity |
33747 |
4.2.1.2 | Iron |
one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form |
33747, 33768 |
4.2.1.2 | Iron |
[4Fe-4S]-cluster containing heterodimeric enzyme |
745719 |
4.2.1.2 | Mg2+ |
10fold activation of isoform Fum2 |
749006 |
4.2.1.2 | Mg2+ |
2 mM, slight stimulation |
678771 |