Information on EC 5.4.99.B25 - tRNA pseudouridine54 synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.B25
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tRNA pseudouridine54 synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tRNA uridine54 = tRNA pseudouridine54
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine54 uracil mutase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine54
tRNA pseudouridine54
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine54
tRNA pseudouridine54
show the reaction diagram
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild-type and mutant enzymes, expression in Escherichia coli. Wild-type enzyme can produce both pseudouridine54 and pseudouridine55 in Escherichia coli tRNAs
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C106A/C109A
-
decrease in tRNA pseudouridine54 synthase activity, no decrease in tRNA pseudouridine55 synthase activity
D275A
-
the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity
D277A
-
decrease in tRNA pseudouridine54 synthase activity and low decrease in tRNA pseudouridine55 synthase activity
H376A/R377A
-
decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity
I412A
-
decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity
K413A
-
decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity
L440A
-
the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity
R273A
-
decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity
Y339A
-
the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology