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Literature summary for 5.4.99.B25 extracted from

  • Joardar, A.; Jana, S.; Fitzek, E.; Gurha, P.; Majumder, M.; Chatterjee K, Geisler M, Gupta R.
    Role of forefinger and thumb loops in production of Psi54 and Psi55 in tRNAs by archaeal Pus10 (2013), RNA, 19, 1279-1294.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes, expression in Escherichia coli. Wild-type enzyme can produce both pseudouridine54 and pseudouridine55 in Escherichia coli tRNAs Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
C106A/C109A decrease in tRNA pseudouridine54 synthase activity, no decrease in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
D275A the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
D277A decrease in tRNA pseudouridine54 synthase activity and low decrease in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
H376A/R377A decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
I412A decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
K413A decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
L440A the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
R273A decrease in tRNA pseudouridine54 synthase activity and in tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii
Y339A the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q60346
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Methanocaldococcus jannaschii DSM 2661 Q60346
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine54 the enzyme produces pseudouridine54 not only in tRNAPhe, but also in nearly all tRNAs. The enzyme also exhibts tRNA pseudouridine55 synthase activity. The forefinger loop (reminiscent of that of RluA) and an Arg and a Tyr residue of archaeal Pus10 as critical determinants for its tRNA pseudouridine54 synthase, but not for its tRNA pseudouridine55 activity. A Leu residue, in addition to the catalytic Asp, is essential for both activities. Archaeal Pus10 proteins must use a different mechanism of recognition for tRNA pseudouridine55 than for the recognition of pseudouridine54. It is proposed that archaeal Pus10 uses two distinct mechanisms for substrate uridine recognition and binding. No mutation is detected that affects only tRNA pseudouridine54 synthase activity, both mechanisms for archaeal Pus10 activities must share some common features Methanocaldococcus jannaschii tRNA pseudouridine54
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 ?
tRNA uridine54 the enzyme produces pseudouridine54 not only in tRNAPhe, but also in nearly all tRNAs. The enzyme also exhibts tRNA pseudouridine55 synthase activity. The forefinger loop (reminiscent of that of RluA) and an Arg and a Tyr residue of archaeal Pus10 as critical determinants for its tRNA pseudouridine54 synthase, but not for its tRNA pseudouridine55 activity. A Leu residue, in addition to the catalytic Asp, is essential for both activities. Archaeal Pus10 proteins must use a different mechanism of recognition for tRNA pseudouridine55 than for the recognition of pseudouridine54. It is proposed that archaeal Pus10 uses two distinct mechanisms for substrate uridine recognition and binding. No mutation is detected that affects only tRNA pseudouridine54 synthase activity, both mechanisms for archaeal Pus10 activities must share some common features Methanocaldococcus jannaschii DSM 2661 tRNA pseudouridine54
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 ?

Synonyms

Synonyms Comment Organism
MjPus10
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 Methanocaldococcus jannaschii