Literature summary for 5.4.99.B25 extracted from

Gurha, P.; Gupta, R.
Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA (2008), RNA, 14, 2521-2527.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Pyrococcus furiosus
-	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-
Pyrococcus furiosus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNA uridine54	the enzyme can function as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently. Unlike bacterial TruB and yeast Pus4, archaeal Pus10 does not require a U54*A58 reverse Hoogstein base pair and pyrimidine at position 56 to convert tRNA uridine55 to pseudouridine55. Although the T(PSI)PSI-arm of tRNA is a good substrate for both pseudouridine54 and pseudouridine55 synthesis by Mj-Pus10, the production of pseudouridine55 is more efficient than that of pseudouridine54 in this substrate. This contrasts with full-size tRNA substrates, where syntheses of both pseudouridines appear to be equally efficient	Methanocaldococcus jannaschii	tRNA pseudouridine54	-	?
tRNA uridine54	the bifunctional enzyme can act as synthase for both tRNA pseudouridine54 and pseudouridine55. The two modifications seem to occur independently	Pyrococcus furiosus	tRNA pseudouridine54	-	?

Synonyms

Synonyms	Comment	Organism
Mj-Pus10	-	Methanocaldococcus jannaschii
PSI55 synthase	-	Methanocaldococcus jannaschii
Pus10	-	Pyrococcus furiosus
Pus10	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)