Information on EC 5.4.99.9 - UDP-galactopyranose mutase

New: Word Map on EC 5.4.99.9
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
5.4.99.9
-
RECOMMENDED NAME
GeneOntology No.
UDP-galactopyranose mutase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
group transfer
-
-
intramolecular
-
isomerization
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
Galactose metabolism
-
-
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
-
-
O-antigen building blocks biosynthesis (E. coli)
-
-
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis
-
-
UDP-galactofuranose biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-galactopyranose furanomutase
A flavoenzyme which generates UDP-alpha-D-glactofuranose required for cell wall formation in bacteria, fungi, and protozoa.
CAS REGISTRY NUMBER
COMMENTARY hide
174632-18-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain RD6.13
-
-
Manually annotated by BRENDA team
strain RD6.13
-
-
Manually annotated by BRENDA team
strain N2 variant Bristol
-
-
Manually annotated by BRENDA team
no activity in Homo sapiens
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-fluoro-deoxy-UDP-galactopyranose
2-fluoro-deoxy-UDP-galactofuranose
show the reaction diagram
-
-
-
?
UDP-(6-deoxy-6-fluoro)-D-galactofuranose
?
show the reaction diagram
-
-
-
-
?
UDP-(6-deoxy-6-fluoro)-D-galactofuranose
UDP-(6-deoxy-6-fluoro)-D-galactopyranose
show the reaction diagram
-
-
-
-
r
UDP-2-amino-2-deoxy-D-galactopyranose
?
show the reaction diagram
-
-
-
-
?
UDP-2-deoxy-2-fluoro-D-galactofuranose
?
show the reaction diagram
-
-
-
-
?
UDP-2-deoxy-2-fluoro-D-galactopyranose
?
show the reaction diagram
-
-
-
-
?
UDP-2-fluoro-galactofuranose
?
show the reaction diagram
-
-
-
-
?
UDP-3''-deoxy-3''-fluoro-D-galactopyranose
UDP-3''-deoxy-3''-fluoro-D-galactofuranose
show the reaction diagram
-
-
-
-
r
UDP-3-deoxy-3-fluoro-D-galactofuranose
?
show the reaction diagram
UDP-3-deoxy-3-fluoro-D-galactopyranose
?
show the reaction diagram
-
-
-
-
?
UDP-6-deoxy-6-fluoro-D-galactopyranose
?
show the reaction diagram
-
-
-
-
?
UDP-6-deoxy-D-galactopyranose
?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactopyranose
UDP-alpha-D-galactofuranose
show the reaction diagram
UDP-beta-L-arabinofuranose
?
show the reaction diagram
-
-
-
-
?
UDP-D-galactofuranose
UDP-D-galactopyranose
show the reaction diagram
UDP-D-galactopyranose
UDP-D-galactofuranose
show the reaction diagram
UDP-galactofuranose
UDP-galactopyranose
show the reaction diagram
UDP-galactopyranose
UDP-galactofuranose
show the reaction diagram
UDP-L-arabinofuranose
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-2-deoxy-2-amino-alpha-D-galactopyranose
UDP-N-acetyl-2-deoxy-2-amino-alpha-D-galactofuranose
show the reaction diagram
Uridine 5'-(trihydrogen diphosphate) P'-alpha-D-galactopyranosyl ester
Uridine 5'-(trihydrogen diphosphate) P'-alpha-D-galactofuranosyl ester
show the reaction diagram
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactopyranose
UDP-alpha-D-galactofuranose
show the reaction diagram
UDP-D-galactopyranose
UDP-D-galactofuranose
show the reaction diagram
UDP-galactopyranose
UDP-galactofuranose
show the reaction diagram
UDP-N-acetyl-2-deoxy-2-amino-alpha-D-galactopyranose
UDP-N-acetyl-2-deoxy-2-amino-alpha-D-galactofuranose
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FADH2
-
reduced FAD is required for activity. Reconstitution of apoenzyme with other FAD analogues, e.g. with methoxy-FAD, trifluoromethyl-FAD, or chloro-FAD, which are less effective than FAD, overview
NAD(P)+
-
-
NAD(P)H
NADH
-
or NADPH required
NADPH
-
or NADH required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,3S,4R,7R,8S)-3-hydroxymethyl-2,6-dioxa-bicyclo-[2.2.2]-octane-7,8-diol
-
17% inhibition at 4 mM
(2R,3S,4S,5S,2'R,3'S,4'S,5'S)-2,2'-butane-1,4-diylbis[5-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxypyrrolidinium] dichloride
-
-
(2S,3S,4S,5R)-2-[(1S)-1,2-dihydroxyethyl]-5-propylpyrrolidine-3,4-diol
-
-
(2S,3S,4S,5R)-2-[(1S)-1,2-dihydroxyethyl]-5-[(1E)-prop-1-en-1-yl]pyrrolidine-3,4-diol
-
-
(2Z)-2-(2-chloro-4-hydroxy-5-nitrobenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one
-
comparison with inhibition of Mycobacterium tuberculosis enzyme; dissociation constant 0.0053 mM, comparison with inhibition of Klebsiella pneumoniae enzyme
(4-chlorophenyl)-[1-(4-chlorophenyl)-3-hydroxy-5-methyl-1H-pyrazol-4-yl]-methanone
(4E)-4-(4-chloro-3-nitrobenzylidene)-1-(3,4-dichlorophenyl)pyrazolidine-3,5-dione
-
comparison with inhibition of Mycobacterium tuberculosis enzyme; dissociation constant 0.0049 mM, comparison with inhibition of Klebsiella pneumoniae enzyme
(Z)-N-((E)-5-(5-nitro-2-oxoindolin-3-ylidene)-4-oxothiazolidin-2-ylidene) benzenesulfonamide
-
-
-
1(R)-1,4-dideoxy-1-C-3-[(ethyl)(uridin-5'-yl)phosphono]-2-propen-1-yl-1,4-imino-D-galactitol
-
25 mM, 52% residual activity
1(R)-1,4-dideoxy-1-C-3-[(uridin-5'-yl)phosphono]-2-propen-1-yl-1,4-imino-D-galactitol
-
2.5 mM, 43% residual activity
1(R)-1-C-allyl-1,4-dideoxy-1,4-imino-D-galactitol
-
25 mM, 61% residual activity
1,4-anhydro-beta-D-galactopyranose (1,5-anhydro-alpha-D-galactofuranose)
-
15% inhibition at 4 mM
1,4-bis-[1(R)-1,4-dideoxy-1,4-imino-D-galactit-1-yl]-2-butene
-
2.5 mM, 50% residual activity
2-(([2-(4-bromophenyl)-1,3-dioxo-2,3-dihydro-1H-isoindol-5-yl]carbonyl)amino)benzoic acid
-
comparison with inhibition of Mycobacterium tuberculosis enzyme
2-(2-(4-bromophenyI)-1, 3-dioxoisoindolin-5-carboxamido) benzoic acid
-
-
-
2-[(5E)-5-(3-bromobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoic acid
-
comparison with inhibition of Mycobacterium tuberculosis enzyme; dissociation constant 0.0094 mM, comparison with inhibition of Klebsiella pneumoniae enzyme
2-[(5E)-5-(4-bromobenzylidene)-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoic acid
-
dissociation constant 0.0093 mM, comparison with inhibition of Klebsiella pneumoniae enzyme
2-[(5Z)-5-[(3-chlorophenyl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoic acid
-
-
2-[5-(3-bromo-benzylidene)-4-oxo-2-thioxo-thiazolidin-3-yl]-3-phenyl-propionic acid
-
-
2-[[2-(4-bromo-phenyl)-1,3-dioxo-2,3-dihydro-1H-isoindole-5-carbonyl]-amino]-benzoic acid
-
-
2-[[4-(3,4-dichlorophenyl)-1,3-thiazol-2-yl]amino]-3-(3-iodophenyl)propanoic acid
-
-
3-(4-iodophenyl)-2-[4-(3,4-dichlorophenyl)-thiazol-2-ylamino]-propionic acid
Ethambutol
N-(4-[4-[8-([[3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]carbamothioyl]amino)octyl]phenyl]-2,3-dihydro-1,3-thiazol-2-yl)-4-chlorophenylalanine
-
-
N-[4-oxo-5-(2-oxo-1,2-dihydro-indol-3-ylidene)-thiazolidin-2-ylidene]-benzenesulfonamide
-
-
Quinine
Sodium borohydride
-
-
Sodium cyanoborohydride
-
-
UDP-(1(1')E)-1_-fluoro-exo-glycal-D-galactofuranose
-
less than 10% inhibition at 1 mM. Time-dependent inactivation proceeds via two-electron processes
UDP-(1(1')Z)-1'-fluoro-exo-glycal-D-galactofuranose
-
less than 10% inhibition at 1 mM. Time-dependent inactivation proceeds via two-electron processes
UDP-C-alpha-D-galactofuranose
-
91% inhibition at 1 mM
UDP-C-alpha-D-galactopyranose
-
36% inhibition at 1 mM
UDP-C-beta-D-galactopyranose
-
8% inhibition at 1 mM
UDP-CH2-Galp
UDP-D-glucose
-
poor inhibitor of UGM
UDP-[1(1')Z]-exo-glycal-D-galactopyranose
-
42% inhibition at 1 mM
uridine diphosphate
-
-
uridine-5'-diphospho-(N-fluoresceinisothiocyano)hexanolamine
-
-
additional information
-
fluorinated substrate analogues under non-reducing conditions
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-Deazariboflavin
-
specific activity 29fold higher than of control without illumination
NAD(P)H
-
enzyme activation mechanism, overview
NADP(H)
Sodium dithionite
-
20 mM
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24
UDP-(6-deoxy-6-fluoro)-D-galactofuranose
1.14
UDP-2-amino-2-deoxy-D-galactopyranose
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.065
UDP-2-deoxy-2-fluoro-D-galactofuranose
-
-
0.203
UDP-2-deoxy-2-fluoro-D-galactopyranose
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.065
UDP-2-fluoro-galactofuranose
-
-
0.861
UDP-3-deoxy-3-fluoro-D-galactofuranose
0.28
UDP-3-deoxy-3-fluoro-D-galactopyranose
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.2
UDP-6-deoxy-6-fluoro-D-galactopyranose
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
3.15
UDP-6-deoxy-D-galactopyranose
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.022 - 0.607
UDP-alpha-D-galactopyranose
0.016 - 0.194
UDP-D-galactofuranose
0.043 - 1.002
UDP-D-galactopyranose
0.02 - 0.19
UDP-galactofuranose
0.022 - 0.055
UDP-galactopyranose
0.6
UDP-L-arabinofuranose
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.4
UDP-(6-deoxy-6-fluoro)-D-galactofuranose
1.4
UDP-2-amino-2-deoxy-D-galactopyranose
Klebsiella pneumoniae
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.033
UDP-2-deoxy-2-fluoro-D-galactofuranose
Escherichia coli
-
-
0.0007
UDP-2-deoxy-2-fluoro-D-galactopyranose
Klebsiella pneumoniae
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.033
UDP-2-fluoro-galactofuranose
Mycobacterium tuberculosis
-
-
5.7
UDP-3-deoxy-3-fluoro-D-galactofuranose
0.9
UDP-3-deoxy-3-fluoro-D-galactopyranose
Klebsiella pneumoniae
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
3.5
UDP-6-deoxy-6-fluoro-D-galactopyranose
Klebsiella pneumoniae
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
42.2
UDP-6-deoxy-D-galactopyranose
Klebsiella pneumoniae
-
in 50 mM MOPS, 10 mM sodium dithionite, 2 mM MgCl2 pH 7.4, at 37C
0.0224 - 13.4
UDP-alpha-D-galactopyranose
1.5 - 72
UDP-D-galactofuranose
0.13 - 35.3
UDP-D-galactopyranose
27
UDP-galactofuranose
66
UDP-galactopyranose
Deinococcus radiodurans
-
wild type enzyme, in 50 mM sodium phosphate buffer pH 7.0
12
UDP-L-arabinofuranose
Escherichia coli
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34
UDP-(6-deoxy-6-fluoro)-D-galactofuranose
Mycobacterium tuberculosis
-
-
14473
0.41
UDP-2-fluoro-galactofuranose
Mycobacterium tuberculosis
-
-
40133
0.00013 - 0.205
UDP-alpha-D-galactopyranose
57 - 650
UDP-D-galactofuranose
1230
UDP-galactofuranose
Mycobacterium tuberculosis
-
-
12721
1180
UDP-galactopyranose
Deinococcus radiodurans
-
wild type enzyme, in 50 mM sodium phosphate buffer pH 7.0
12722
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
2-[5-(3-bromo-benzylidene)-4-oxo-2-thioxo-thiazolidin-3-yl]-3-phenyl-propionic acid
-
-
0.0046
2-[[2-(4-bromo-phenyl)-1,3-dioxo-2,3-dihydro-1H-isoindole-5-carbonyl]-amino]-benzoic acid
-
-
0.017
N-[4-oxo-5-(2-oxo-1,2-dihydro-indol-3-ylidene)-thiazolidin-2-ylidene]-benzenesulfonamide
-
-
0.0013
uridine-5'-diphospho-(N-fluoresceinisothiocyano)hexanolamine
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 0.035
(2Z)-2-(2-chloro-4-hydroxy-5-nitrobenzylidene)[1,3]thiazolo[3,2-a]benzimidazol-3(2H)-one
0.044 - 0.062
(4-chlorophenyl)-[1-(4-chlorophenyl)-3-hydroxy-5-methyl-1H-pyrazol-4-yl]-methanone
0.004 - 0.041
(4E)-4-(4-chloro-3-nitrobenzylidene)-1-(3,4-dichlorophenyl)pyrazolidine-3,5-dione
0.0046
2-(([2-(4-bromophenyl)-1,3-dioxo-2,3-dihydro-1H-isoindol-5-yl]carbonyl)amino)benzoic acid
Klebsiella pneumoniae
-
pH 7.0, 37C
0.0016 - 0.065
2-[(5E)-5-(3-bromobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoic acid
0.0072 - 0.037
3-(4-iodophenyl)-2-[4-(3,4-dichlorophenyl)-thiazol-2-ylamino]-propionic acid
0.0035
N-(4-[4-[8-([[3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]carbamothioyl]amino)octyl]phenyl]-2,3-dihydro-1,3-thiazol-2-yl)-4-chlorophenylalanine
Mycobacterium tuberculosis
-
-
0.411 - 0.495
UDP-CH2-Galp
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Escherichia coli (strain K12)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Trypanosoma cruzi (strain CL Brener)
Trypanosoma cruzi (strain CL Brener)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
-
gel filtration
92000
-
gel filtration
228600
-
calculated from amino acid sequence
275000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 57200, about, sequence calculation
decamer
homodimer
homotetramer
-
4 * 55000, SDS-PAGE; 4 * 57152, calculated from amino acid sequence
monomer
tetramer
-
dimer-of-dimers tetramer in solution, the C-terminal helix of domain 1 and residue Arg133 in AfUGM form intersubunit hydrogen bonds in the AfUGM tetramer, while long side chains protruding from a helix of domain 2 likely prevent formation of the intersubunit 4-helix bundle that stabilizes the AfUGM tetramer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis, two crystal forms, hexagonal and triclinic
-
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 500 nl 15 mg/ml protein solution with 500 nl reservoir solution, containing 0.1 M HEPES, pH 7.5, 20% PEG 3350, 0.4 M ammonium sulfate, 4% formamide, and equilibration against 0.07 ml reservoir solution, for enzyme complex crystals, the enzyme is incubated for 10 min on ice with various substrate/inhibitor/reducing ligands 10 mM UDP-Galp, 10 mM UDP, 5 mM UDP-glucose, 5-20 mM sodium dithionite, respectively, before crystallization, 2 days, X-ray diffraction structure determination and analysis at 3.25 A resolution. Incorporation of selenomethionine is achieved, but the resulting crystals do not allow solution of the phase problem
-
wild-type and mutant enzyme in complex with substrate UDP-alpha-D-galactopyranose or with inhibitor UDP, microbatch method at room temperature, for the substrate complex crystals: 10 mg/ml protein in 50 mM Tris, pH 8.0, 5 mM DTT, with 10 mM UDP-alpha-D-galactopyranose, and 10 mM dithionite, for the inhibitor complex crystals: 10 mg/ml protein in 25 mM Tris malonate, pH 8.0, and 10 mM UDP, for mutant enzyme-substrate complexes: 10 mg/ml protein in 25 mM Tris malonate, pH 8.0, with 15 mM UDP-alpha-D-galactopyranose, mixing of equal volumes of protein solution (with or without ligand) and crystallization solution and overlaid with oil, X-ray diffraction structure determination and analysis at 2.3-3.15 A resolution
-
crystal structure analysis
-
in complex with UDP-CH2-Galp, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 6.5), 0.2 M LiCl, and 28% (w/v) polyethylene glycol 6000
in complex with UDP-galactopyranose and UDP and uridine diphosphate, microbatch under oil method, using 0.1 M HEPES, pH 6.5, 0.2 M lithium chloride and 28% (w/v) PEG 6000
-
in complex with UDP-galactopyranose, microbatch-under-oil method, using 0.1 M HEPES pH 7.0, 0.2 M LiCl, and 20% (w/v) PEG 6000
-
crystal structure analysis
-
FAD is located in a cleft lined with conserved residues
vapour-diffusion method in hanging drops, orthorhombic, space group P21 with a: 71.12 A, b: 58.42 A, c: 96.38 A, beta: 96.38
-
crystal structure analysis
-
in complex with UDP-D-glucose
-
in the (active) reduced state, with FAD, crystal structure at 2.2 A resolution, with FADH-, crystal structure at 2.25 A resolution
-
crystal structure analysis
-
in the oxidized state, with FAD, crystal structure at 2.25 A resolution
-
substrate-bound enzyme in oxidized and reduced forms
crystal structure analysis
-
crystal structure analysis, molecular dynamics simulations and modeling, overview
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HQ20 column chromatography and HP-20 column chromatography
-
HQ20 column chromatography and HP-20 hydrophobic interaction chromatography column
-
nickel immobilized affinity chromatography and DEAE column chromatography
-
nickel immobilized metal affinity chromatography, DEAE column chromatography, and purification with HaloLink resin
-
Protino Ni-IDA 1000 column chromatography
recombinant C-terminally His6-tagged enzyme from Escherichia coi strain BL21 StarTM (DE3) by nickel affinity chromatography
-
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS or B893 (DE3) pLysS by glutathione affinity chroatography and gel filtration
-
the recombinant enzyme
-
the recombinant enzyme from Escherichia coli
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21T1 and BL21(DE3) cells
-
expressed in Escherichia coli BL21T1 cells
-
expressed in Escherichia coli C43 cells
expressed in Escherichia coli DH5alpha cell
-
expressed in Escherichia coli strain strain MFF1
-
expressed in Escherichia coli Tuner cells
-
expression in Escherichia coli
expression in Escherichia coli (BL21(DE3))
-
expression in Escherichia coli (ER2566)
-
expression in Escherichia coli with a His-tag
expression in Escherichia coli, His-tagged protein
-
expression with His-tag in Escherichai coli
-
gene glf, overexpression of C-terminally His6-tagged enzyme in Escherichia coi strain BL21 StarTM (DE3)
-
overexpression in Escherichia coli
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, and in strain B893 (DE3) pLysS for the selnomethionine-labeled enzyme
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R182A
-
site-directed mutagenesis, the mutant is almost inactive
R182K
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R327A
-
site-directed mutagenesis, inactive mutant
R327K
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D351A
-
mutation of conserved residue of putative active site cleft. 4% of wild-type activity
E301A
-
mutation of conserved residue of putative active site cleft. 21% of wild-type activity
R174A
-
no catalytic activity
R280A
-
mutation of conserved residue of putative active site cleft. No catalytic activity
W156A
-
lower activity than wild-type enzyme
W156Y
-
lower activity than wild-type enzyme
W70F/W290F
Y151F
-
lower activity than wild-type enzyme
Y155F
-
7% of wild-type activity
Y181F
-
lower activity than wild-type enzyme
Y185F
-
11% of wild-type activity
Y311F
-
lower activity than wild-type enzyme
Y314F
-
5% of wild-type activity
Y346F
-
lower activity than wild-type enzyme
Y349F
-
6% of wild-type activity
R176A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R327A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y317F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y395F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y429F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
high-throughput fluorescence polarization assay for indentification of inhibitors of enzyme and homologues
drug development
medicine
-
the UDP-galactopyranose mutase is an appealing adjunct therapeutic target in combination with other drugs against Aspergillus fumigatus
synthesis
-
production of uridine 5'-(trihydrogen diphosphate) P'-alpha-D-galactofuranosyl ester, a precursor required for the formation of the lipopolysaccharide O-antigen of Klebsiella pneumoniae serotype O1
Show AA Sequence (1216 entries)
Please use the Sequence Search for a certain query.