Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.9 extracted from

  • Qi, J.; Oppenheimer, M.; Sobrado, P.
    Fluorescence polarization binding assay for Aspergillus fumigatus virulence factor UDP-galactopyranose mutase (2011), Enzyme Res., 2011, 513905.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.9

Application

Application Comment Organism
drug development inhibitors of the enzyme that block the biosynthesis of UDP-galactofuranose can lead to novel chemotherapeutics for treating Aspergillus fumigatus-related diseases Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
(Z)-N-((E)-5-(5-nitro-2-oxoindolin-3-ylidene)-4-oxothiazolidin-2-ylidene) benzenesulfonamide
-
 Aspergillus fumigatus
2-(2-(4-bromophenyI)-1, 3-dioxoisoindolin-5-carboxamido) benzoic acid
-
 Aspergillus fumigatus
UDP competitive Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information high-affinity binding to the enzyme is only obtained with the chromophore 5-carboxytetramethylrhodamine, i.e. TAMRA, linked to UDP by either 2 or 6 carbons, 6times less when UDP is linked to fluorescein, kinetics, overview Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactopyranose Aspergillus fumigatus
-
 UDP-alpha-D-galactofuranose
-
 r

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information development of a fluorescence polarization binding assay for the Aspergillus fumigatus enzyme, evaluation, overview Aspergillus fumigatus ?
-
 ?
UDP-alpha-D-galactopyranose
-
 Aspergillus fumigatus UDP-alpha-D-galactofuranose
-
 r

Synonyms

Synonyms Comment Organism
UGM
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function UDP-galactopyranose mutase catalyzes the isomerization of UDP-galactopyranose to UDP-galactofuranose, the biosynthetic precursor of galactofuranose residues, which are essential components of the cell wall and play an important role in Aspergillus fumigatus virulence Aspergillus fumigatus