Information on EC 4.2.2.9 - pectate disaccharide-lyase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.2.9
-
RECOMMENDED NAME
GeneOntology No.
pectate disaccharide-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
eliminative cleavage of 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate from the reducing end of pectate, i.e. de-esterified pectin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose and glucuronate interconversions
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-galacturonan reducing-end-disaccharide-lyase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37290-87-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Clostridium multifermentans
-
-
-
Manually annotated by BRENDA team
strain W2
-
-
Manually annotated by BRENDA team
rumen bacterium, strain ATCC 19207
-
-
Manually annotated by BRENDA team
subsp. carotovora
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methyl-di-D-galacturonate
?
show the reaction diagram
-
alpha-(1-4)-linked, 36% of the activity with di-D-galacturonate
-
?
1-methyl-tri-D-galacturonate
?
show the reaction diagram
2-methyl-di-D-galacturonate
?
show the reaction diagram
-
alpha-(1-4)-linked, 35% of the activity with di-D-galacturonate
-
?
2-methyl-tri-D-galacturonate
?
show the reaction diagram
-
alpha-(1-4)-linked, 19% of the activity with tri-D-galacturonate or 1-methyl-tri-D-galacturonate
-
?
3-methyl-tri-D-galacturonate
?
show the reaction diagram
DELTA4,5-unsaturated tri-D-galacturonate
?
show the reaction diagram
-
alpha-(1-4)-linked, low activity with PelX
-
?
di-D-galacturonate
?
show the reaction diagram
-
alpha-(1-4)-linked
-
?
galacturonic acid
?
show the reaction diagram
-
-
-
-
?
heptagalacturonate
?
show the reaction diagram
better substrate than polygalacturonate
-
-
?
hexagalacturonic acid
?
show the reaction diagram
pectic acid
4,5-unsaturated digalacturonic acid
show the reaction diagram
pectin
?
show the reaction diagram
-
-
-
-
?
pentagalacturonic acid
?
show the reaction diagram
poly-D-galacturonic acid
?
show the reaction diagram
-
pectin from citrus
-
?
polygalacturonate
unsaturated digalacturonate
show the reaction diagram
tetragalacturonic acid
?
show the reaction diagram
tri-D-galacturonate
?
show the reaction diagram
trigalacturonic acid
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Clostridium multifermentans
-
inducible
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
Clostridium multifermentans
-
stimulates
Cu2+
weak activation
Fe2+
activates, 85% of maximal activity in 0.1 M TES, pH 7.4, 0.1 mM Fe2+
K+
-
slight stimulation
Ni2+
weak activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-ethyl-3-(3-dimethylamino-propyl) carbodiimide
-
weak inhibitor
Al3+
-
inhibitory
Ba2+
-
slight inhibition
Ca2+
-
inhibitory
diethylenetriaminepentaacetate
-
-
Fe2+
-
inhibitory
hydroxyethylenediaminetriacetate
-
mixed type inhibition
-
Mg2+
-
slight inhibition
Mn2+
-
inhibitory
N-bromosuccinimide
-
complete inhibition
trans-1,2-cyclohexanediaminetetraacetate
-
-
Zn2+
-
inhibitory
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dithiodipyridine
-
-
5,5-dithio-bis 2-nitrobenzoic acid
-
-
beta-mercaptoethanol
-
-
diethyl dicarbonate
-
-
dithiothreitol
-
-
L-tryptophan
-
-
PelN
presence of PelN has an synergistic effect on PelX activity
-
triethylenetetramine
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0032
oligogalacturonate
-
-
0.0013
polygalacturonate I
-
-
-
0.0016
polygalacturonate II
-
-
-
0.0054
trigalacturonate
-
-
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.311
-
fractionated enzyme in supernatant fluid, no activity in crude cell extract
4.1
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
85% of maximal activity in 0.1 M TES, pH 7.4, 0.1 mM Fe2+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
Clostridium multifermentans
-
pH 5: no activity, pH 6.0: 15% of maximal activity, pH 7.0: 40% of maximal activity, pH 8.5: maximal activity
7 - 9
Clostridium multifermentans
-
pH 7: about 40% of maximal activity, pH 9: about 85% of maximal activity
8 - 9
-
up to 55C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Clostridium multifermentans
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32400
-
equilibrium centrifugation
35000
-
SDS-PAGE, mass spectrometry
38000
-
1 * 38000, SDS-PAGE
39000
-
gel filtration
54000
-
gel filtration
76000
-
x * 76000, SDS-PAGE
76938
x * 76938, calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
-
contains about 13% carbohydrate
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
-
2C, 72 h, relatively stable
34115
5 - 9
-
at 50-55C
669558
6
Clostridium multifermentans
-
38C, most stable at
34111
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 7.0, 10 min, stable below
60
-
pH 7.0, 10 min, 85% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ stablizes
Mn2+ stablizes
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Clostridium multifermentans
-
ultrafiltration and three-step chromatographic procedures, Sephacryl S-100 column, DEAE-Sepharose ion-exchange column, Sephadex G-50 column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
industry
-
degumming and retting of fiber crops and pre-treatment of pectic waste water from fruit juice industries
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