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Literature summary for 4.2.2.9 extracted from
- Purification and characterization of a novel pectinase from Acrophialophora nainiana with emphasis on its physicochemical properties (2006), J. Biotechnol., 123, 33-42.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dithiodipyridine | - |
Acrophialophora nainiana |  |
| 5,5-dithio-bis 2-nitrobenzoic acid | - |
Acrophialophora nainiana | |
| beta-mercaptoethanol | - |
Acrophialophora nainiana | |
| diethyl dicarbonate | - |
Acrophialophora nainiana | |
| dithiothreitol | - |
Acrophialophora nainiana | |
| L-cysteine | - |
Acrophialophora nainiana | |
| L-tryptophan | - |
Acrophialophora nainiana | |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | degumming and retting of fiber crops and pre-treatment of pectic waste water from fruit juice industries | Acrophialophora nainiana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide | weak inhibitor | Acrophialophora nainiana | |
| Al3+ | inhibitory | Acrophialophora nainiana | |
| Ca2+ | inhibitory | Acrophialophora nainiana | |
| Cu2+ | inhibitory | Acrophialophora nainiana | |
| Fe2+ | inhibitory | Acrophialophora nainiana | |
| Mn2+ | inhibitory | Acrophialophora nainiana | |
| N-bromosuccinimide | complete inhibition | Acrophialophora nainiana | |
| Zn2+ | inhibitory | Acrophialophora nainiana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no effect | Acrophialophora nainiana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE, mass spectrometry | Acrophialophora nainiana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acrophialophora nainiana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultrafiltration and three-step chromatographic procedures, Sephacryl S-100 column, DEAE-Sepharose ion-exchange column, Sephadex G-50 column chromatography | Acrophialophora nainiana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galacturonic acid | - |
Acrophialophora nainiana | ? | - |
? | |
| pectin | - |
Acrophialophora nainiana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Acrophialophora nainiana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-pectate lyase | - |
Acrophialophora nainiana |
| exopolymethylgalacturonate lyase | - |
Acrophialophora nainiana |
| PECI | extracellular pectinase | Acrophialophora nainiana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acrophialophora nainiana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | - |
Acrophialophora nainiana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 70 | - |
Acrophialophora nainiana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Acrophialophora nainiana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | up to 55°C | Acrophialophora nainiana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | at 50-55°C | Acrophialophora nainiana |
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