Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,2'-dithiodipyridine | - |
Acrophialophora nainiana | |
5,5-dithio-bis 2-nitrobenzoic acid | - |
Acrophialophora nainiana | |
beta-mercaptoethanol | - |
Acrophialophora nainiana | |
diethyl dicarbonate | - |
Acrophialophora nainiana | |
dithiothreitol | - |
Acrophialophora nainiana | |
L-cysteine | - |
Acrophialophora nainiana | |
L-tryptophan | - |
Acrophialophora nainiana |
Application | Comment | Organism |
---|---|---|
industry | degumming and retting of fiber crops and pre-treatment of pectic waste water from fruit juice industries | Acrophialophora nainiana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylamino-propyl) carbodiimide | weak inhibitor | Acrophialophora nainiana | |
Al3+ | inhibitory | Acrophialophora nainiana | |
Ca2+ | inhibitory | Acrophialophora nainiana | |
Cu2+ | inhibitory | Acrophialophora nainiana | |
Fe2+ | inhibitory | Acrophialophora nainiana | |
Mn2+ | inhibitory | Acrophialophora nainiana | |
N-bromosuccinimide | complete inhibition | Acrophialophora nainiana | |
Zn2+ | inhibitory | Acrophialophora nainiana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no effect | Acrophialophora nainiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE, mass spectrometry | Acrophialophora nainiana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acrophialophora nainiana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration and three-step chromatographic procedures, Sephacryl S-100 column, DEAE-Sepharose ion-exchange column, Sephadex G-50 column chromatography | Acrophialophora nainiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galacturonic acid | - |
Acrophialophora nainiana | ? | - |
? | |
pectin | - |
Acrophialophora nainiana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Acrophialophora nainiana |
Synonyms | Comment | Organism |
---|---|---|
exo-pectate lyase | - |
Acrophialophora nainiana |
exopolymethylgalacturonate lyase | - |
Acrophialophora nainiana |
PECI | extracellular pectinase | Acrophialophora nainiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acrophialophora nainiana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Acrophialophora nainiana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | - |
Acrophialophora nainiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acrophialophora nainiana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | up to 55°C | Acrophialophora nainiana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | at 50-55°C | Acrophialophora nainiana |