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Literature summary for 4.2.2.9 extracted from

  • Macmillan, J.D.; Vaughn, R.H.
    Purification and properties of a polygalacturonic acid-trans-eliminase produced by Clostridium multifermentans (1964), Biochemistry, 3, 564-572.
    View publication on PubMed
Search for more literature for 4.2.2.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Clostridium multifermentans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Clostridium multifermentans
Mn2+ stimulates Clostridium multifermentans
Sr2+ stimulates Clostridium multifermentans

Organism

Organism UniProt Comment Textmining
Clostridium multifermentans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Clostridium multifermentans

Reaction

Reaction Comment Organism Reaction ID
(1,4-alpha-D-galacturonosyl)n = (1,4-alpha-D-galacturonosyl)n-2 + 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate trans-elimination mechanism Clostridium multifermentans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with pectin of high methoxyl content Clostridium multifermentans ?
-
 ?
polygalacturonate
-
 Clostridium multifermentans unsaturated digalacturonate the major end product is O-(4-deoxy-beta-L-threo-hexopyranos-4-enyluronic acid)-(1,4)-D-galacturonic acid ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Clostridium multifermentans

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7: about 40% of maximal activity, pH 9: about 85% of maximal activity Clostridium multifermentans