Information on EC 4.2.1.40 - glucarate dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.40
-
RECOMMENDED NAME
GeneOntology No.
glucarate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
-
-
D-glucarate degradation I
-
-
D-glucarate degradation II
-
-
Metabolic pathways
-
-
degradation of sugar acids
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glucarate hydro-lyase (5-dehydro-4-deoxy-D-glucarate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9059-02-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Paracolobactrum sp.
-
-
-
Manually annotated by BRENDA team
A
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-deoxy-4-fluoro-D-glucarate
?
show the reaction diagram
D-glucarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
D-Glucarate
?
show the reaction diagram
D-glucarate
D-idarate
show the reaction diagram
D-idarate
D-glucarate
show the reaction diagram
D-idarate
L-glucarate
show the reaction diagram
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
show the reaction diagram
D-Glucarate
?
show the reaction diagram
D-idarate
L-glucarate
show the reaction diagram
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
less than one-third as effective as Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-tartrate
-
2.5 mM, 58% inhibition
(-)-tartrate
-
2.5 mM, 5% inhibition
4-deoxy-D-glucarate
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5-Amino-1H-tetrazole
-
-
Co2+
-
high concentrations
D-idaric acid
-
-
galactaric acid
-
-
meso-tartrate
-
2.5 mM, 31% inhibition
Mn2+
-
high concentrations
p-chloromercuribenzene sulfonate
xylarohydroxamate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 1.42
D-glucarate
0.016
L-idarate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 44
4-deoxy-4-fluoro-D-glucarate
0.00033 - 35
D-glucarate
0.00025 - 35
L-idarate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay method
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
Tris-maleate buffer
7.5 - 8.5
-
Tris-HCl buffer
7.5
-
barbital buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
activity assay
PDB
SCOP
CATH
ORGANISM
UNIPROT
Actinobacillus succinogenes (strain ATCC 55618 / 130Z)
Burkholderia lata (strain ATCC 17760 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Pseudomonas mendocina (strain ymp)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
determined by SDS-PAGE
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method
crystal structure at 2.3 A resolution
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
-
moderately stable
33840
7.5
-
most stable near
33840
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D366A
-
reduced kcat for D-glucarate and L-idarate
D366N
-
reduced kcat for D-glucarate and 3fold reduced kcat for L-idarate
H339A
-
reduced kcat for D-glucarate and L-idarate
H339N
-
reduced kcat for D-glucarate and L-idarate
H339Q
-
reduced kcat for D-glucarate and L-idarate
K207Q
-
reduced kcat for D-glucarate and L-idarate
K207R
-
reduced kcat for D-glucarate and L-idarate
N341D
-
do not catalyze the dehydrofluorination of 4-deoxy-4-fluoro-D-glucarate
N341L
-
catalyzes the dehydrofluorination of 4-deoxy-4-fluoro-D-glucarate
Y150F
-
significant reduction of kcat for D-glucarate and L-idarate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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