Literature summary for 4.2.1.40 extracted from

Hubbard, B.K.; Koch, M.; Palmer, D.R.J.; Babbitt, P.C.; Gerlt, J.A.
Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli (1998), Biochemistry, 37, 14369-14375.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	L-idarate	-	Escherichia coli
0.06	-	D-glucarate	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	sequence alignment	Bacillus subtilis
additional information	-	sequence alignment	Escherichia coli
additional information	-	sequence alignment	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Escherichia coli	-	-	-
Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucarate	-	Bacillus subtilis	3-deoxy-L-threo-2-hexulosarate + H2O	-	?
D-glucarate	-	Escherichia coli	3-deoxy-L-threo-2-hexulosarate + H2O	i.e. 4-deoxy-5-ketoglucarate	?
D-glucarate	-	Pseudomonas putida	3-deoxy-L-threo-2-hexulosarate + H2O	i.e. 4-deoxy-5-ketoglucarate	?
D-glucarate	-	Escherichia coli CR63MA	3-deoxy-L-threo-2-hexulosarate + H2O	i.e. 4-deoxy-5-ketoglucarate	?
D-glucarate	epimerization	Escherichia coli	D-idarate	epimerization	r
D-glucarate	epimerization	Pseudomonas putida	D-idarate	epimerization	r
D-idarate	epimerization	Escherichia coli	D-glucarate	epimerization	r
D-idarate	epimerization	Pseudomonas putida	D-glucarate	epimerization	r
L-idarate	-	Escherichia coli	3-deoxy-L-threo-2-hexulosarate	i.e. 4-deoxy-5-ketoglucarate	?
L-idarate	-	Pseudomonas putida	3-deoxy-L-threo-2-hexulosarate	i.e. 4-deoxy-5-ketoglucarate	?
L-idarate	-	Escherichia coli CR63MA	3-deoxy-L-threo-2-hexulosarate	i.e. 4-deoxy-5-ketoglucarate	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20	-	D-glucarate	-	Escherichia coli
20	-	L-idarate	-	Escherichia coli

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Release 2023.1 (January 2023)