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Literature summary for 4.2.1.40 extracted from

  • Jeffcoat, R.
    Chemical modification and kinetic studies on glucarate hydro-lyase from a species of Pseudomonas A (1972), Eur. J. Biochem., 25, 515-523.
    View publication on PubMed
Search for more literature for 4.2.1.40

Inhibitors

Inhibitors Comment Organism Structure
(+)-tartrate 2.5 mM, 58% inhibition Pseudomonas sp.
(-)-tartrate 2.5 mM, 5% inhibition Pseudomonas sp.
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Pseudomonas sp.
5-Amino-1H-tetrazole
-
 Pseudomonas sp.
EDTA 1 mM, 94.5% inhibition Pseudomonas sp.
meso-tartrate 2.5 mM, 31% inhibition Pseudomonas sp.
p-chloromercuribenzene sulfonate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.42
-
 D-glucarate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 A
-
Pseudomonas sp. A
-
 A
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucarate
-
 Pseudomonas sp. 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?
D-glucarate
-
 Pseudomonas sp. A 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
 Pseudomonas sp.