Information on EC 4.2.1.109 - methylthioribulose 1-phosphate dehydratase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
4.2.1.109
-
RECOMMENDED NAME
GeneOntology No.
methylthioribulose 1-phosphate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-methyl-5-thio-D-ribulose 1-phosphate = 5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dehydration
-, O31668
-
dehydration
Bacillus subtilis 168 (trp C2)
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
-
Metabolic pathways
-
S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation I
-
Yang cycle
-
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5-thio-D-ribulose-1-phosphate 4-hydro-lyase [5-(methylthio)-2,3-dioxopentyl-phosphate-forming]
This enzyme forms part of the methionine-salvage pathway.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1-PMT-ribulose dehydratase
-
-
5-Methylthioribulose-1-phosphate
O31668
-
5-Methylthioribulose-1-phosphate
Bacillus subtilis 168 (trp C2)
O31668
-
-
5-methylthioribulose-1-phosphate dehydratase
P47095
-
MtnB
O31668
-
MtnB
Bacillus subtilis 168 (trp C2)
O31668
-
-
MTRu-1-P dehydratase
O31668
-
MTRu-1-P dehydratase
Bacillus subtilis 168 (trp C2)
O31668
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 168 (trp C2)
TrEMBL
Manually annotated by BRENDA team
Bacillus subtilis 168 (trp C2)
strain 168 (trp C2)
TrEMBL
Manually annotated by BRENDA team
gene Yjr024cp by homology comparison
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-
-
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-
-
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-, O31668
-
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-, O31668
-
the reaction product 5-(methylthio)-2,3-dioxopentyl phosphate is so labile that its chemical structure could not be isolated and analyzed
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
P47095
enzyme of the methionine salvage pathway
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-
the enzyme forms part of the methionine-salvage pathway
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
Bacillus subtilis 168 (trp C2)
O31668
-
-, the reaction product 5-(methylthio)-2,3-dioxopentyl phosphate is so labile that its chemical structure could not be isolated and analyzed
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-, O31668
-
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
P47095
enzyme of the methionine salvage pathway
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
-
the enzyme forms part of the methionine-salvage pathway
-
-
?
S-methyl-5-thio-D-ribulose 1-phosphate
5-(methylthio)-2,3-dioxopentyl phosphate + H2O
show the reaction diagram
Bacillus subtilis 168 (trp C2)
O31668
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-, O31668
MtnB requires metal ions for catalysis, but it is unclear what kind of metal ions are bound to the MtnB purified from Escherichia coli cells. Considering that FucA/RibE/RhuA-related enzymes require the zinc ion for optimal catalysis under physiological conditions and that recombinant class II aldolase enzymes expressed in Escherichia coli cells are almost all of the Zn2+ form, purified MtnB might also be of the Zn2+ form
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0089
-
S-methyl-5-thio-D-ribulose 1-phosphate
-, O31668
25C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
16.5
-
S-methyl-5-thio-D-ribulose 1-phosphate
-, O31668
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
8
-, O31668
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
10.5
-, O31668
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-, O31668
at 55C the enzyme loses most of its activity
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
15
55
-, O31668
at 55C the enzyme loses most of its activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Aquifex aeolicus (strain VF5)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
90000
-
-, O31668
without His-tag, 4 * 23800, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homotetramer
-, O31668
4 * 23800
homotetramer
Bacillus subtilis 168 (trp C2)
-
4 * 23800
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
homogeneity, using the His-tag, the His-tag is cleaved with thrombin
-, O31668
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed as (His)6-tagged soluble protein in Escherichia BL21 (DE3), vector pET15b
-, O31668