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Literature summary for 4.2.1.109 extracted from

  • Mary, C.; Duek, P.; Salleron, L.; Tienz, P.; Bumann, D.; Bairoch, A.; Lane, L.
    Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway (2012), PLoS ONE, 7, e52877.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information transient silencing of the enzyme in HeLa cells. Stable knockdown of the enzyme specifically affects growth in 5-methylthioadenosine and depletes intracellular levels of methionine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required by the enzyme protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96GX9 gene mtnB
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
5'-methylthioribulose-1-phosphate dehydratase
-
Homo sapiens
APIP
-
Homo sapiens

General Information

General Information Comment Organism
malfunction the enzyme depletion specifically impairs the capacity of cells to grow in media where methionine is replaced by 5-methylthioadenosine. Knockdown of the enzyme specifically affects the recycling of methionine, and mutation of three potential phosphorylation sites does not affect the enzyme activity whereas mutation of the potential zinc binding site completely abrogates it Homo sapiens
metabolism the enzyme catalyzes the dehydratase step in the methionine salvage pathway Homo sapiens
physiological function the enzyme is involved in the methionine salvage pathway that recycles the sulfur-containing metabolite 5-methylthioadenosine to methionine Homo sapiens