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Literature summary for 4.2.1.109 extracted from

  • Nakano, T.; Ohki, I.; Yokota, A.; Ashida, H.
    MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila (2013), PLoS ONE, 8, e67385.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene mtnBD, identification of the domains responsible for catalyzing the four reactions from the enzyme, overview. Recombinant expression of His-tagged proteins of full-length enzyme and the MtnB domain in Escherichia coli BL21(DE3) Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-5-thio-D-ribulose 1-phosphate Tetrahymena thermophila activity of the MtnB domain 5-(methylthio)-2,3-dioxopentyl phosphate + H2O
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?

Organism

Organism UniProt Comment Textmining
Tetrahymena thermophila I7MAL0 gene mtnBD
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-methyl-5-thio-D-ribulose 1-phosphate activity of the MtnB domain Tetrahymena thermophila 5-(methylthio)-2,3-dioxopentyl phosphate + H2O
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?

Synonyms

Synonyms Comment Organism
MtnBD
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Tetrahymena thermophila
MTRu-1-P dehydratase/enolase
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Tetrahymena thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Tetrahymena thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Tetrahymena thermophila

General Information

General Information Comment Organism
evolution the MtnB domain of Tetrahymena thermophila MtnBD acquired the new function to catalyze both the dehydratase and enolase reactions through evolutionary gene mutations, rather than fusion of MSP genes Tetrahymena thermophila
metabolism the MtnBD fusion enzyme in Tetrahymena thermophila catalyzes two of the sequential reactions from the dehydratase to dioxygenase steps in the methionine salvage pathway: 5-methylthioribulose-1-phosphate is converted to 2-oxo-4-methylthiobutyrate, the methionine precursor, by four steps, namely dehydratase, enolase, phosphatase, and dioxygenase reactions. The MtnB domain catalyzes dehydration and enolization and the MtnD domain catalyzes dioxygenation, the phosphatase is distinct from the enzyme. Methionine salvage pathway, overview Tetrahymena thermophila
additional information identification of the domains responsible for catalyzing the four reactions from the enzyme, overview Tetrahymena thermophila