Cloned (Comment) | Organism |
---|---|
gene mtnBD, identification of the domains responsible for catalyzing the four reactions from the enzyme, overview. Recombinant expression of His-tagged proteins of full-length enzyme and the MtnB domain in Escherichia coli BL21(DE3) | Tetrahymena thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5-thio-D-ribulose 1-phosphate | Tetrahymena thermophila | activity of the MtnB domain | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetrahymena thermophila | I7MAL0 | gene mtnBD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5-thio-D-ribulose 1-phosphate | activity of the MtnB domain | Tetrahymena thermophila | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtnBD | - |
Tetrahymena thermophila |
MTRu-1-P dehydratase/enolase | - |
Tetrahymena thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Tetrahymena thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tetrahymena thermophila |
General Information | Comment | Organism |
---|---|---|
evolution | the MtnB domain of Tetrahymena thermophila MtnBD acquired the new function to catalyze both the dehydratase and enolase reactions through evolutionary gene mutations, rather than fusion of MSP genes | Tetrahymena thermophila |
metabolism | the MtnBD fusion enzyme in Tetrahymena thermophila catalyzes two of the sequential reactions from the dehydratase to dioxygenase steps in the methionine salvage pathway: 5-methylthioribulose-1-phosphate is converted to 2-oxo-4-methylthiobutyrate, the methionine precursor, by four steps, namely dehydratase, enolase, phosphatase, and dioxygenase reactions. The MtnB domain catalyzes dehydration and enolization and the MtnD domain catalyzes dioxygenation, the phosphatase is distinct from the enzyme. Methionine salvage pathway, overview | Tetrahymena thermophila |
additional information | identification of the domains responsible for catalyzing the four reactions from the enzyme, overview | Tetrahymena thermophila |