Information on EC 3.7.1.5 - acylpyruvate hydrolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.7.1.5
-
RECOMMENDED NAME
GeneOntology No.
acylpyruvate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 3-acylpyruvate + H2O = a carboxylate + pyruvate
show the reaction diagram
acts on formylpyruvate, 2,4-dioxopentanoate, 2,4-dioxohexanoate and 2,4-dioxoheptanoate
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-C bond
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Microbial metabolism in diverse environments
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Tyrosine metabolism
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SYSTEMATIC NAME
IUBMB Comments
3-acylpyruvate acylhydrolase
Acts on formylpyruvate, 2,4-dioxopentanoate, 2,4-dioxohexanoate and 2,4-dioxoheptanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
54004-67-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
isoform 2
UniProt
Manually annotated by BRENDA team
activity occurs also in strains OH101, MI214, and MI214/1
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-
Manually annotated by BRENDA team
activity occurs also in strains OH101, MI214, and MI214/1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dioxoheptanoate + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-dioxohexanoate + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-dioxovalerate + H2O
?
show the reaction diagram
-
-
-
-
?
3-formylpyruvate + H2O
formate + pyruvate
show the reaction diagram
-
-
-
ir
3-fumarylpyruvate + H2O
fumarate + pyruvate
show the reaction diagram
acetylpyruvate + H2O
acetate + pyruvate
show the reaction diagram
acylpyruvate + H2O
fatty acid + pyruvate
show the reaction diagram
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-
-
?
fumarylpyruvate + H2O
fumarate + pyruvate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
0.1 mM
Ni2+
-
0.1 mM
Zn2+
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0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CN-
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62% activity increase at 0.1 mM
EDTA
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32% activity increase at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
2,4-Dioxoheptanoate
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-
0.007
2,4-Dioxohexanoate
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0.011
2,4-Dioxovalerate
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0.002
3-formylpyruvate
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0.0046
Acetylpyruvate
pH 7.4, 22°C, recombinant wild-type FAHD1
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.014
pH 7.4, 22°C, purified recombinant wild-type FAHD1, substrate acetylpyruvate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
-
assay at
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high expression level of FAHD1
Manually annotated by BRENDA team
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high expression level of FAHD1
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, SDS-PAGE
72000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FAHD1 partially from human umbilical vein cells, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dioalysis, cation exchange chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression of FAHD1 in human umbilical vein cells, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
growth on 3-hydroxybenzoate and 2,5-dihydroxybenzoate leads to the induction of fumarylpyruvate hydrolase
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D102A
site-directed mutagenesis
R106A
site-directed mutagenesis
additional information
Show AA Sequence (146 entries)
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