Cloned (Comment) | Organism |
---|---|
expression in BL21(DE3) Escherichia coli LysS cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method at 18°C, crystal structure of the enzyme (FAHD1) protein in complex with its cofactor Mg2+, cocrystallization with oxalate and high resolution for the enzyme (FAHD1) in complex with oxalate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E33A | significant reduction in oxaloacetate decarboxylase activity and complete abrogation of the acylpyruvate hydrolase activity | Homo sapiens |
H30A | significant reduction in oxaloacetate decarboxylase activity and complete abrogation of the acylpyruvate hydrolase activity | Homo sapiens |
K123A | oxaloacetate decarboxylase activity and acylpyruvate hydrolase activity are abolished | Homo sapiens |
additional information | mutation of amino acids H30, E33 and K123 each had discernible influence on the acylpyruvate hydrolase and/or oxaloacetate decarboxylase activity of FAHD1, suggesting distinct catalytic mechanisms for both activities | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | competitive inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0405 | - |
Acetylpyruvate | pH 7.4, 25°C, wild-type enzyme | Homo sapiens | |
0.0421 | - |
Acetylpyruvate | pH 7.4, 25°C, mutant enzyme E33A | Homo sapiens | |
0.0819 | - |
Acetylpyruvate | pH 7.4, 25°C, mutant enzyme H30A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P587 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylpyruvate + H2O | bi-functional enzyme displaying both acylpyruvate hydrolase and oxaloacetate decarboxylase activity | Homo sapiens | acetate + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAH domain-containing protein 1 | - |
Homo sapiens |
FAHD1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important for mitochondrial function. It acts antagonistically to pyruvate carboxylase, a key metabolic enzyme | Homo sapiens |