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Literature summary for 3.7.1.5 extracted from

  • Pircher, H.; Straganz, G.D.; Ehehalt, D.; Morrow, G.; Tanguay, R.M.; Jansen-Duerr, P.
    Identification of human fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) as a novel mitochondrial acylpyruvase (2011), J. Biol. Chem., 286, 36500-36508.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.7.1.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of FAHD1 in human umbilical vein cells, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS Homo sapiens

Protein Variants

Protein Variants Comment Organism
D102A site-directed mutagenesis Homo sapiens
additional information lentiviral shRNA knockdown of FAHD1 in human umbilical vein cells Homo sapiens
R106A site-directed mutagenesis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
 Acetylpyruvate pH 7.4, 22°C, recombinant wild-type FAHD1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-
mitochondrion
-
 Homo sapiens 5739
-
additional information not in cytosol and nucleus Mus musculus
-
-
additional information not in cytosol and nucleus Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Mus musculus
Mg2+ required for activity Homo sapiens
additional information Mg2+ cannot properly be substituted by Ca2+ or Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6P587 isoform 2
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FAHD1 partially from human umbilical vein cells, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dioalysis, cation exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney high expression level of FAHD1 Mus musculus
-
liver high expression level of FAHD1 Mus musculus
-
additional information ubiquitous expression of FAHD1 in murine tissues Mus musculus
-
umbilical vein
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
 pH 7.4, 22°C, purified recombinant wild-type FAHD1, substrate acetylpyruvate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylpyruvate + H2O
-
 Mus musculus acetate + pyruvate
-
 ?
acetylpyruvate + H2O
-
 Homo sapiens acetate + pyruvate
-
 ?
fumarylpyruvate + H2O
-
 Mus musculus fumarate + pyruvate
-
 ?
fumarylpyruvate + H2O
-
 Homo sapiens fumarate + pyruvate
-
 ?
additional information fumaroylacetoacetate hydrolase domain-containing protein 1, FAHD1, exhibits acylpyruvase activity and hydrolyzes fumarylpyruvate and acetylpyruvate in vitro, several other structural derivatives are not or poorly hydrolyzed Mus musculus ?
-
 ?
additional information fumaroylacetoacetate hydrolase domain-containing protein 1, FAHD1, exhibits acylpyruvase activity and hydrolyzes fumarylpyruvate and acetylpyruvate in vitro, several other structural derivatives, e.g. 3-methylacetopyruvate and acetylactone, are not or poorly hydrolyzed Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Homo sapiens
? x * 25000-30000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
acylpyruvate hydrolase
-
 Mus musculus
acylpyruvate hydrolase
-
 Homo sapiens
FAHD1
-
 Mus musculus
FAHD1
-
 Homo sapiens
fumaroylacetoacetate hydrolase domain-containing protein 1
-
 Mus musculus
fumaroylacetoacetate hydrolase domain-containing protein 1
-
 Homo sapiens
More cf. EC 3.7.1.2 Mus musculus
More cf. EC 3.7.1.2 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution fumaroylacetoacetate hydrolase domain-containing protein 1, FAHD1, is part of the FAH protein superfamily Mus musculus
evolution fumaroylacetoacetate hydrolase domain-containing protein 1, FAHD1, is part of the FAH protein superfamily Homo sapiens
additional information Asp102 and Arg106 ate important residues for catalysis Mus musculus
additional information Asp102 and Arg106 ate important residues for catalysis Homo sapiens