Information on EC 3.7.1.23 - maleylpyruvate hydrolase

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The expected taxonomic range for this enzyme is: Pseudomonas alcaligenes

EC NUMBER
COMMENTARY hide
3.7.1.23
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RECOMMENDED NAME
GeneOntology No.
maleylpyruvate hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-maleylpyruvate + H2O = maleate + pyruvate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,5-xylenol and 3,5-xylenol degradation
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gentisate degradation II
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Tyrosine metabolism
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SYSTEMATIC NAME
IUBMB Comments
(2Z)-4,6-dioxohept-2-enedioate acylhydrolase
The enzyme, characterized from the bacterium Pseudomonas alcaligenes NCIMB 9867, catalyses the hydrolysis of 3-maleylpyruvate, the ring-cleavage product of gentisate. The enzyme can also act on a number of maleylpyruvate derivatives, such as (2E)-2-methyl-4,6-dioxohept-2-enedioate and (2E)-3-methyl-4,6-dioxohept-2-enedioate. Activated by Mn2+. May be identical to EC 3.7.1.5, acylpyruvate hydrolase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-maleylpyruvate + H2O
maleate + pyruvate
show the reaction diagram
6-bromomaleylpyruvate + H2O
6-bromomaleate + pyruvate
show the reaction diagram
6-chloromaleylpyruvate + H2O
6-chloromaleate + pyruvate
show the reaction diagram
6-ethylmaleylpyruvate + H2O
6-ethylmaleate + pyruvate
show the reaction diagram
6-fluoromaleylpyruvate + H2O
6-fluoromaleate + pyruvate
show the reaction diagram
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100% activity compared to 3-maleylpyruvate
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?
6-methylmaleylpyruvate + H2O
6-methylmaleate + pyruvate
show the reaction diagram
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110% activity compared to 3-maleylpyruvate
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?
6-propylmaleylpyruvate + H2O
6-propylmaleate + pyruvate
show the reaction diagram
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10% activity compared to 3-maleylpyruvate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-maleylpyruvate + H2O
maleate + pyruvate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cd2+
complete inhibition at 1 mM
Co2+
complete inhibition at 1 mM
Cu2+
complete inhibition at 1 mM
Ni2+
complete inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014 - 0.0199
3-maleylpyruvate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.17
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crude extract, pH and temperature not specified in the publication
8.8
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after 52fold purification, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, with 50% (v/v) glycerol, 20 days, 38% loss of activity
-80C, with 50% (v/v) glycerol, 50 days, 73% loss of activity
4C, with 5% (v/v) glycerol, 10 days, 50% loss of activity
4C, with 5% (v/v) glycerol, 40 days, 79% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sephadex G-150 gel filtration, DEAE-Sephadex A50 gel filtration, and triethylanilnoethyl-cellulose column chromatography
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Ni Sepharose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
neither 3-hydroxybenzoate nor gentisate can induce the synthesis of maleylpyruvate hydrolase II