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Literature summary for 3.7.1.23 extracted from
- Purification and some properties of maleylpyruvate hydrolase and fumarylpyruvate hydrolase from Pseudomonas alcaligenes (1980), J. Bacteriol., 143, 70-77.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
3-maleylpyruvate | pH and temperature not specified in the publication | Pseudomonas alcaligenes |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Pseudomonas alcaligenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
| 77000 | - |
gel filtration | Pseudomonas alcaligenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-maleylpyruvate + H2O | Pseudomonas alcaligenes | 100% activity | maleate + pyruvate | - |
? | |
| 3-maleylpyruvate + H2O | Pseudomonas alcaligenes NCIB 9867 | 100% activity | maleate + pyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas alcaligenes | - |
- |
- |
| Pseudomonas alcaligenes NCIB 9867 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sephadex G-150 gel filtration, DEAE-Sephadex A50 gel filtration, and triethylanilnoethyl-cellulose column chromatography | Pseudomonas alcaligenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
crude extract, pH and temperature not specified in the publication | Pseudomonas alcaligenes |
| 8.8 | - |
after 52fold purification, pH and temperature not specified in the publication | Pseudomonas alcaligenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-maleylpyruvate + H2O | 100% activity | Pseudomonas alcaligenes | maleate + pyruvate | - |
? | |
| 3-maleylpyruvate + H2O | 100% activity | Pseudomonas alcaligenes NCIB 9867 | maleate + pyruvate | - |
? | |
| 6-bromomaleylpyruvate + H2O | 130% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-bromomaleate + pyruvate | - |
? | |
| 6-bromomaleylpyruvate + H2O | 130% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes NCIB 9867 | 2-bromomaleate + pyruvate | - |
? | |
| 6-chloromaleylpyruvate + H2O | 130% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-chloromaleate + pyruvate | - |
? | |
| 6-chloromaleylpyruvate + H2O | 130% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes NCIB 9867 | 2-chloromaleate + pyruvate | - |
? | |
| 6-ethylmaleylpyruvate + H2O | 60% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-ethylmaleate + pyruvate | - |
? | |
| 6-ethylmaleylpyruvate + H2O | 60% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes NCIB 9867 | 2-ethylmaleate + pyruvate | - |
? | |
| 6-fluoromaleylpyruvate + H2O | 100% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-fluoromaleate + pyruvate | - |
? | |
| 6-methylmaleylpyruvate + H2O | 110% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-methylmaleate + pyruvate | - |
? | |
| 6-propylmaleylpyruvate + H2O | 10% activity compared to 3-maleylpyruvate | Pseudomonas alcaligenes | 2-propylmaleate + pyruvate | - |
? | |
| additional information | no activity against either fumarylpyruvate, maleylacetoacetate, or fumarylacetoacetate | Pseudomonas alcaligenes | ? | - |
? | |
| additional information | no activity against either fumarylpyruvate, maleylacetoacetate, or fumarylacetoacetate | Pseudomonas alcaligenes NCIB 9867 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPH11 | - |
Pseudomonas alcaligenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.7 | - |
Pseudomonas alcaligenes |
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