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Literature summary for 3.7.1.23 extracted from
- HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867 (2013), Appl. Environ. Microbiol., 79, 1044-1047.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas alcaligenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes |  |
| Co2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
| Cu2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
| Ni2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0199 | - |
3-maleylpyruvate | at pH 7.6 and 30°C | Pseudomonas alcaligenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | addition of Mn2+ at 0.05 or 0.1 mM enhances the enzyme activity by 30% | Pseudomonas alcaligenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
| 77000 | - |
gel filtration | Pseudomonas alcaligenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-maleylpyruvate + H2O | Pseudomonas alcaligenes | - |
maleate + pyruvate | - |
? | |
| 3-maleylpyruvate + H2O | Pseudomonas alcaligenes NCIMB 9867 | - |
maleate + pyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas alcaligenes | Q0QFQ3 | - |
- |
| Pseudomonas alcaligenes NCIMB 9867 | Q0QFQ3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose column chromatography | Pseudomonas alcaligenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, with 50% (v/v) glycerol, 20 days, 38% loss of activity | Pseudomonas alcaligenes |
| -80°C, with 50% (v/v) glycerol, 50 days, 73% loss of activity | Pseudomonas alcaligenes |
| 4°C, with 5% (v/v) glycerol, 10 days, 50% loss of activity | Pseudomonas alcaligenes |
| 4°C, with 5% (v/v) glycerol, 40 days, 79% loss of activity | Pseudomonas alcaligenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes | maleate + pyruvate | - |
? | |
| 3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes NCIMB 9867 | maleate + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hbzF | - |
Pseudomonas alcaligenes |
| maleylpyruvate hydrolase | - |
Pseudomonas alcaligenes |
| Mph | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas alcaligenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Pseudomonas alcaligenes |
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Release 2023.1 (January 2023)
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