Information on EC 3.5.3.2 - guanidinoacetase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.3.2
-
RECOMMENDED NAME
GeneOntology No.
guanidinoacetase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
guanidinoacetate + H2O = glycine + urea
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amidine hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
guanidinoacetate amidinohydrolase
Requires Mn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-92-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-guanidinopropionate + H2O
3-aminopapanoate + urea
show the reaction diagram
4-guanidinobutyrate + H2O
4-aminobutanoate + urea
show the reaction diagram
-
-
-
-
?
guanidinoacetate + H2O
glycine + urea
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanidinoacetate + H2O
glycine + urea
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
CaCl2 1 mM in addition to 0.1 mM MnCl2, relative activity 134%
Fe2+
-
0.1 mM slight activation
Mg2+
-
MgSO4 1 mM in addition to 0.1 mM MnCl2, relative activity 127%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
8-hydroxyquinoline
-
-
ATP
-
1.0 mM 50% inhibition
Co2+
-
1 mM, plus 0.1 mM Mn2+ complete inhibition
Cu2+
-
1 mM plus 0.1 mM Mn2+ complete inhibition
DTNB
-
inhibits the reaction slightly
EDTA
-
complete loss of activity, fully restored with Mn2+
glycine
-
competitive inhibition
n-butylguanidine
-
-
n-Decylamine
-
-
n-dodecylamine
-
-
n-octylamine
-
-
n-propylguanidine
-
-
Ni2+
-
1 mM, plus 0.1 mM Mn2+ complete inhibition
p-chloromercuribenzoate
Zn2+
-
1 mM, plus 0.1 mM Mn2+ complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
4-aminobutyrate
-
5 mM, relative activity 106%
4-Guanidinobutyrate
-
5 mM, relative activity 109%
5,5'-dithiobis(2-nitrobenzoate)
-
0.1 mM, relative activity 138%
dithiothreitol
-
-
N-ethylmaleimide
-
0.1 mM, relative activity 117%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.1 - 16
Guanidinoacetate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
54.1 - 60.2
Guanidinoacetate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.39
-
ATCC 12633, GA induced
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 9.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
4 * 70000, SDS-PAGE
150000
-
gel filtration
160000
-
gel filtration, BioGel P-200
163000
-
gel filtration
165000
-
gel filtration, disc gel electrophoreisis
281000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
colorless needles
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10
-
stable in this range
209437
6 - 10.5
-
stable in this range at 30°C for 1 h
209441
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 20 mM Tris-HCl buffer, pH 8, 1.0 mM 2-mercaptoethanol, 0.1 mM MnCl2, 6months without loss of activity
-
4°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 1.0 mM MnCl2, 70% saturated ammonium sulfate, crystalline enzyme can be stored for at least 2 months without serious loss in activity, without added Mn2+ or 2-mercaptoethanol activity rapidly lost with a half-life of about 3 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine