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Literature summary for 3.5.3.2 extracted from
- Purification and some properties of guanidinoacetate amidinohydrolase produced by Corynebacterium sp. (1986), J. Ferment. Technol., 64, 29-36.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, activity 102% | Corynebacterium sp. |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzymic determination of guanidinoacetic acid in urine, guanidinoacetic acid decreases in patients with kidney disease or patients who have undergone kidney transplantation, low amount of guanidinoacetic acid in urine is an indicator of renal dysfunction | Corynebacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Corynebacterium sp. | |
| 1,10-phenanthroline | - |
Flavobacterium sp. | |
| 8-hydroxyquinoline | - |
Corynebacterium sp. | |
| Fe2+ | 1 mM, plus 0.1 mM Mn2+ partial inhibition, relative activity 40% | Corynebacterium sp. | |
| glycine | competitive inhibition | Corynebacterium sp. | |
| Hg2+ | - |
Corynebacterium sp. | |
| Iodine | - |
Corynebacterium sp. | |
| n-butylguanidine | - |
Corynebacterium sp. | |
| n-Decylamine | - |
Corynebacterium sp. | |
| n-dodecylamine | - |
Corynebacterium sp. | |
| n-octylamine | - |
Corynebacterium sp. | |
| n-propylguanidine | - |
Corynebacterium sp. | |
| p-chloromercuribenzoate | - |
Corynebacterium sp. | |
| p-chloromercuribenzoate | - |
Flavobacterium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
Guanidinoacetate | - |
Corynebacterium sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Corynebacterium sp. | |
| Ni2+ | - |
Corynebacterium sp. | |
| Zn2+ | restores activity of 1,10-phenanthroline treated enzyme | Corynebacterium sp. | |
| Zn2+ | restores activity of 1,10-phenanthroline treated enzyme | Flavobacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, SDS-PAGE | Corynebacterium sp. |
| 150000 | - |
gel filtration | Corynebacterium sp. |
| 281000 | - |
- |
Flavobacterium sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanidinoacetate + H2O | Pseudomonas putida | - |
glycine + urea | - |
? | |
| guanidinoacetate + H2O | Corynebacterium sp. | - |
glycine + urea | - |
? | |
| guanidinoacetate + H2O | Penicillium roqueforti | - |
glycine + urea | - |
? | |
| guanidinoacetate + H2O | Flavobacterium sp. | - |
glycine + urea | - |
? | |
| guanidinoacetate + H2O | Corynebacterium sp. N-19-1 | - |
glycine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium sp. | - |
N-19-1 | - |
| Corynebacterium sp. N-19-1 | - |
N-19-1 | - |
| Flavobacterium sp. | - |
F. sp. GE-1 | - |
| Penicillium roqueforti | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.8 | - |
- |
Corynebacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-guanidinopropionate + H2O | - |
Corynebacterium sp. | 3-aminopapanoate + urea | - |
? | |
| 3-guanidinopropionate + H2O | - |
Flavobacterium sp. | 3-aminopapanoate + urea | - |
? | |
| 3-guanidinopropionate + H2O | - |
Corynebacterium sp. N-19-1 | 3-aminopapanoate + urea | - |
? | |
| 4-guanidinobutyrate + H2O | - |
Pseudomonas putida | 4-aminobutanoate + urea | - |
? | |
| guanidinoacetate + H2O | - |
Pseudomonas putida | glycine + urea | - |
? | |
| guanidinoacetate + H2O | - |
Corynebacterium sp. | glycine + urea | - |
? | |
| guanidinoacetate + H2O | - |
Penicillium roqueforti | glycine + urea | - |
? | |
| guanidinoacetate + H2O | - |
Flavobacterium sp. | glycine + urea | - |
? | |
| guanidinoacetate + H2O | - |
Corynebacterium sp. N-19-1 | glycine + urea | - |
? | |
| additional information | - |
Flavobacterium sp. | ? | - |
? | |
| additional information | guanidine, methylguanidine, ethylguanidine, n-propylguanidine, L-arginine, N-acetyl-L-arginine, L-argininate, agmatine, creatine, creatinine and guanidinosuccinate are no substrates | Corynebacterium sp. | ? | - |
? | |
| additional information | guanidine, methylguanidine, ethylguanidine, n-propylguanidine, L-arginine, N-acetyl-L-arginine, L-argininate, agmatine, creatine, creatinine and guanidinosuccinate are no substrates | Corynebacterium sp. N-19-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Flavobacterium sp. |
| tetramer | 4 * 38000, SDS-PAGE | Corynebacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Corynebacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Corynebacterium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | stable in this range at 30°C for 1 h | Corynebacterium sp. |
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