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Literature summary for 3.5.3.2 extracted from

  • Yorifuji, T.; Tamai, H.; Usami, H.
    Purification, crystallisation and properties of Mn2+ dependent guanidinoacetate amidinohydrolase from a Pseudomonas (1977), Agric. Biol. Chem., 41, 959-966.
No PubMed abstract available
Search for more literature for 3.5.3.2

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol together with Mn2+ Pseudomonas sp.
4-aminobutyrate 5 mM, relative activity 106% Pseudomonas sp.
4-Guanidinobutyrate 5 mM, relative activity 109% Pseudomonas sp.
5,5'-dithiobis(2-nitrobenzoate) 0.1 mM, relative activity 138% Pseudomonas sp.
dithiothreitol
-
 Pseudomonas sp.
N-ethylmaleimide 0.1 mM, relative activity 117% Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
colorless needles Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
ATP 1.0 mM 50% inhibition Pseudomonas sp.
Co2+ 1 mM, plus 0.1 mM Mn2+ complete inhibition Pseudomonas sp.
Cu2+ 1 mM plus 0.1 mM Mn2+ complete inhibition Pseudomonas sp.
EDTA complete loss of activity, fully restored with Mn2+ Pseudomonas sp.
Fe2+ 1 mM, plus 0.1 mM Mn2+ partial inhibition, relative activity 40% Pseudomonas sp.
Ni2+ 1 mM, plus 0.1 mM Mn2+ complete inhibition Pseudomonas sp.
p-chloromercuribenzoate
-
 Flavobacterium sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.
Zn2+ 1 mM, plus 0.1 mM Mn2+ complete inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.1
-
 Guanidinoacetate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ CaCl2 1 mM in addition to 0.1 mM MnCl2, relative activity 134% Pseudomonas sp.
Co2+
-
 Flavobacterium sp.
Co2+ 0.1 mM slight activation Pseudomonas sp.
Fe2+ 0.1 mM slight activation Pseudomonas sp.
Mg2+ MgSO4 1 mM in addition to 0.1 mM MnCl2, relative activity 127% Pseudomonas sp.
Mn2+ dependent, most potent activator Pseudomonas sp.
Ni2+ 0.1 mM slight activation Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 4 * 38000, SDS-PAGE Pseudomonas sp.
160000
-
 gel filtration, BioGel P-200 Pseudomonas sp.
163000
-
 gel filtration Pseudomonas sp.
165000
-
 gel filtration, disc gel electrophoreisis Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanidinoacetate + H2O Pseudomonas sp.
-
 glycine + urea
-
 ?
guanidinoacetate + H2O Pseudomonas putida
-
 glycine + urea
-
 ?
guanidinoacetate + H2O Penicillium roqueforti
-
 glycine + urea
-
 ?
guanidinoacetate + H2O Flavobacterium sp.
-
 glycine + urea
-
 ?
guanidinoacetate + H2O Pseudomonas sp. ATCC 14676
-
 glycine + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Penicillium roqueforti
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. ATCC 14676
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63.7
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-15°C, 20 mM Tris-HCl buffer, pH 8, 1.0 mM 2-mercaptoethanol, 0.1 mM MnCl2, 6months without loss of activity Pseudomonas sp.
4°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 1.0 mM MnCl2, 70% saturated ammonium sulfate, crystalline enzyme can be stored for at least 2 months without serious loss in activity, without added Mn2+ or 2-mercaptoethanol activity rapidly lost with a half-life of about 3 days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanidinoacetate + H2O
-
 Pseudomonas sp. glycine + urea
-
 ?
guanidinoacetate + H2O
-
 Pseudomonas putida glycine + urea
-
 ?
guanidinoacetate + H2O
-
 Penicillium roqueforti glycine + urea
-
 ?
guanidinoacetate + H2O
-
 Flavobacterium sp. glycine + urea
-
 ?
guanidinoacetate + H2O
-
 Pseudomonas sp. ATCC 14676 glycine + urea
-
 ?
additional information
-
 Flavobacterium sp. ?
-
 ?
additional information beta-guanidinopropionate, gamma-guanidinobutyrate, creatine, L-arginine and agmatine are no substrates Pseudomonas sp. ?
-
 ?
additional information beta-guanidinopropionate, gamma-guanidinobutyrate, creatine, L-arginine and agmatine are no substrates Pseudomonas sp. ATCC 14676 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Flavobacterium sp.
tetramer 4 * 38000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54.1
-
 Guanidinoacetate
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 ATP
-
 Pseudomonas sp.