Information on EC 3.4.22.B69 - falcipain-2

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The expected taxonomic range for this enzyme is: Plasmodium falciparum

EC NUMBER
COMMENTARY hide
3.4.22.B69
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
falcipain-2
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The enzyme plays a key role in the hydrolysis of hemoglobin by the parasite Plasmodium falciparum. Preferred cleavage sites display arginine in P1, leucine in P2, and phenylalanine in P1'
show the reaction diagram
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-KLRFSKQ-EDDnp + H2O
?
show the reaction diagram
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?
Abz-KLRSSKQ-EDDnp + H2O
?
show the reaction diagram
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?
Abz-KLRSXKQ-EDDnp + H2O
Abz-KLRS + XKQ-EDDnp
show the reaction diagram
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?
Abz-KLRXSKQ-EDDnp + H2O
Abz-KLR + XSKQ-EDDnp
show the reaction diagram
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?
Abz-KLXSSKQ-EDDnp + H2O
Abz-KLX + SSKQ-EDDnp
show the reaction diagram
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?
Abz-KXRSSKQ-EDDnp + H2O
Abz-KXR + SSKQ-EDDnp
show the reaction diagram
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?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
?
show the reaction diagram
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the peptide corresponds to the Met375 to Ile393 sequence of high molecular weight kininogen. The enzyme preferentially accommodates at the S1 subsite the positively charged residue Arg, followed by Gln. At the P2 position, the enzyme shows a clear preference for the hydrophobic aliphatic residue Leu over Phe. The subsite S3 of FP-2 shows a broad specificity with slight preference of Lys in the P3 position
the generated fragments are KRPPGFSPFR (Lys-bradykinin, 63%), RPPGFSPFR (bradykinin, 30%), and MKRPPGFSPFR (Met-Lys-bradykinin, 7%)
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?
Abz-XLRSSKQ-EDDnp + H2O
Abz-XLR + SSKQ-EDDnp
show the reaction diagram
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?
benzoxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzoxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
Cbz-Phe-Arg-7-amido-4-methylcoumarin + H2O
Cbz-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O
D-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Hemoglobin + H2O
?
show the reaction diagram
high molecular weight kininogen + H2O
Met-Lys-bradykinin + Lys-bradykinin + bradykinin + ?
show the reaction diagram
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?
Z-Leu-Arg-7-amido-4-methylcoumarin + H2O
Z-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
additional information
?
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the falcipain-2 prodomain efficiently inhibits falcipain-2, falcipain-2', berghepain-2, falcipain-3, cathepsin K, cathepsin L, cathepsin B, and cruzain, but it does not inhibit cathepsin C, pepsin, alpha-chymotrypsin, and collagenase
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Hemoglobin + H2O
?
show the reaction diagram
high molecular weight kininogen + H2O
Met-Lys-bradykinin + Lys-bradykinin + bradykinin + ?
show the reaction diagram
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?
additional information
?
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the falcipain-2 prodomain efficiently inhibits falcipain-2, falcipain-2', berghepain-2, falcipain-3, cathepsin K, cathepsin L, cathepsin B, and cruzain, but it does not inhibit cathepsin C, pepsin, alpha-chymotrypsin, and collagenase
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1,1-dioxido-3-oxo-1,2-benzothiazol-2(3H)-yl)(1,3,5-triaza-7-phosphatricyclo[3.3.1.13,7]decane-kappaP)gold
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24% inhibition at 0.02 mM
(4-chloro-2-trifluoromethyl-phenyl)-carbamic acid 5-methyl-2-oxo-1-[(4-oxo-pent-2-enylcarbamoyl)-methyl]-2,3-dihydro-1H-benzo[e][1,4] diazepin-3-methyl ester
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(5Z)-3-(2,4-difluorobenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-(3-methoxybenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-(4-fluorobenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-(4-methylbenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-benzyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-ethyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-methyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-3-[4-(trifluoromethyl)benzyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(5Z)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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(S)-2-(1-(4-benzylpiperidin-1-yl)-1-oxo-3-phenylpropan-2-yl)isoindoline-1,3-dione
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(S)-2-(1-(4-benzylpiperidin-1-yl)-3-methyl-1-oxobutan-2-yl)isoindoline-1,3-dione
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(S)-2-(1-(4-benzylpiperidin-1-yl)-4-methyl-1-oxopentan-2-yl)isoindoline-1,3-dione
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2,2'-((2S,2'S)-piperazine-1,4-diylbis(1-oxo-3-phenylpropane-2,1-diyl))bis(isoindoline-1,3-dione)
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2,2'-((2S,2'S)-piperazine-1,4-diylbis(3-methyl-1-oxobutane-2,1-diyl))bis(isoindoline-1,3-dione)
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2,2'-((2S,2'S)-piperazine-1,4-diylbis(4-methyl-1-oxopentane-2,1-diyl))bis(isoindoline-1,3-dione)
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2,2'-((2S,2'S,3R,3'R)-piperazine-1,4-diylbis(3-methyl-1-oxopentane-2,1-diyl))bis(isoindoline-1,3-dione)
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2-((2S,3S)-1-(4-benzylpiperidin-1-yl)-3-methyl-1-oxopentan-2-yl)isoindoline-1,3-dione
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2-[(2S)-1-(morpholin-4-yl)-1-oxo-3-phenylpropan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-1-oxo-3-phenyl-1-(piperidin-1-yl)propan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-1-oxo-3-phenyl-1-(pyrrolidin-1-yl)propan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-3-methyl-1-(morpholin-4-yl)-1-oxobutan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-3-methyl-1-oxo-1-(piperidin-1-yl)butan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-3-methyl-1-oxo-1-(pyrrolidin-1-yl)butan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-4-methyl-1-(morpholin-4-yl)-1-oxopentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-4-methyl-1-oxo-1-(piperidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S)-4-methyl-1-oxo-1-(pyrrolidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S,3R)-3-methyl-1-(morpholin-4-yl)-1-oxopentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S,3R)-3-methyl-1-oxo-1-(piperidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[(2S,3R)-3-methyl-1-oxo-1-(pyrrolidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
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2-[3-(isobutylamino-methyl)-2-oxo-5-phenyl-2,3-dihydrobenzo[e][1,4]diazepin-1-yl]-N-(4-oxo-pent-2-enyl)-acetamide
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3-adamantyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-(2-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-(3-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-(4-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-(4-cyanophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-(4-methoxyphenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-cyclohexylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-methylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenoxypiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4-dichlorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4-dimethoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,5-ditrifluoromethylbenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-bromobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-chlorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3,4-difluorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-carboxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-fluorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-isopropylbenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-methoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-methylbenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-chlorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-fluorobenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-methoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-methylbenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-trifluoromethylbenzylidene)
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3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-benzylidene-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(4-pyridylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[2-oxo-2-(piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
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3-[4-(benzylsulfamoyl)phenyl]-N-(2,3-dihydro-1,4-benzodioxin-6-yl)propanamide
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less than 5% inhibition at 0.01 mM
3-[4-[(2-phenylethyl)sulfamoyl]phenyl]-N-(5-phenyl-1,3,4-thiadiazol-2-yl)propanamide
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94.5% inhibition at 0.01 mM
3-[4-[(2-phenylethyl)sulfamoyl]phenyl]-N-(pyridin-2-ylmethyl)propanamide
-
10.7% inhibition at 0.01 mM
3-[4-[(2-phenylethyl)sulfamoyl]phenyl]-N-(thiophen-2-ylmethyl)propanamide
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15.2% inhibition at 0.01 mM
3-[4-[(3-chloro-4-fluorophenyl)sulfamoyl]phenyl]-N-(2,3-dihydro-1,4-benzodioxin-6-yl)propanamide
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32.7% inhibition at 0.01 mM
3-[4-[(3-chloro-4-fluorophenyl)sulfamoyl]phenyl]-N-(5-ethyl-1,3,4-thiadiazol-2-yl)propanamide
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45.1% inhibition at 0.01 mM
artemisinin
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artesunate
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auranofin
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30% inhibition at 0.02 mM
benzyl (1-[[(2S)-2-cyanopyrrolidin-1-yl]carbonyl]cyclohexyl)carbamate
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benzyl [1-[(2-oxoethyl)carbamoyl]cyclohexyl]carbamate
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benzyl [1-[(cyanomethyl)amino]-2-methyl-1-oxopropan-2-yl]carbamate
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benzyl [1-[(cyanomethyl)carbamoyl]cyclohexyl]carbamate
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chicken egg white cystatin
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Chloroquine
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Chloroquine diphosphate
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chloro[N-[1-(hydroxy-kappaO)-3-methylbutan-2-yl]pyridine-2-carboxamidato(2-)-kappa2N1,N2]aurate(2-)
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43% inhibition at 0.02 mM
cisplatin
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43% inhibition at 0.02 mM
coproporphyrin
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deuteroporphyrin 3,7,12,17-tetramethyl-21H,23H-porphyrin-2,18-dipropionic acid dihydrochloride
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deuteroporphyrin IX 2,4 (4,2) hydroxyvinyl
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deuteroporphyrin IX 2-vinyl, 4-hydroxymethyl
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dichloro[3-(propan-2-yl)-2-(pyridin-2-yl-kappaN)-4,5-dihydro-1,3-oxazol-3-ium-kappaN]aurate(1-) hexafluorophosphate
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59% inhibition at 0.02 mM
heme
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heme inhibits falcipain-2 activity against both human hemoglobin and chromogenic peptide substrates through a noncompetitive-like mechanism
isohematoporphyrin IX
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leupeptide
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mesoporphyrin IX dimethyl ester
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methyl (2E)-4-[({(3R)-3-[({[4-chloro-2-(trifluoromethyl)phenyl]carbamoyl}oxy)methyl]-2-oxo-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-1-yl}acetyl)amino]but-2-enoate
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N-(1,3-benzothiazol-2-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
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41.5% inhibition at 0.01 mM
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-2-[4-[(2-phenylethyl)sulfamoyl]phenyl]acetamide
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less than 5% inhibition at 0.01 mM
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
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23% inhibition at 0.01 mM
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[4-[(3,4-dimethoxyphenyl)sulfamoyl]phenyl]propanamide
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6.3% inhibition at 0.01 mM
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[4-[(3-methoxyphenyl)sulfamoyl]phenyl]propanamide
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17.9% inhibition at 0.01 mM
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-4-[(2-phenylethyl)sulfamoyl]benzamide
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less than 5% inhibition at 0.01 mM
N-(3,4-dimethoxyphenyl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
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15% inhibition at 0.01 mM
N-(3-hydroxy-4-methoxyphenyl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
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13.6% inhibition at 0.01 mM
N-(4-oxo-pent-2-enyl)-2-(2-oxo-5-phenyl-3-morpholin-1-ylmethyl-2,3-dihydro-benzo[e][1,4]diazepin-1-yl)-acetamide
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N-(4-oxo-pent-2-enyl)-2-(2-oxo-5-phenyl-3-piperidin-1-ylmethyl-2,3-dihydro-benzo[e][1,4] diazepin-1-yl)-acetamide
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80% inhibition at 0.1 mM, no inhibition at 0.03 mM
N-(5-ethyl-1,3,4-thiadiazol-2-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
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46.5% inhibition at 0.01 mM
N-(5-ethyl-1,3,4-thiadiazol-2-yl)-3-[4-[(4-fluorophenyl)sulfamoyl]phenyl]propanamide
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72.9% inhibition at 0.01 mM
N-(cyanomethyl)-1-([[(1S,2S)-2-phenylcyclopropyl]carbonyl]amino)cyclohexanecarboxamide
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N-(cyanomethyl)-1-[(3-cyclopentylpropanoyl)amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[(cyclopentylacetyl)amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(2,3-dimethoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(2-methoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(4-methoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(furan-2-yl)propanoyl]amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(pyridin-3-yl)propanoyl]amino]cyclohexanecarboxamide
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N-(cyanomethyl)-1-[[3-(thiophen-2-yl)propanoyl]amino]cyclohexanecarboxamide
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N-methyl mesoporphyrin
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N-methyl protoporphyrin IX
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potassium bis(1,1-dioxido-3-oxo-1,2-benzothiazol-2(3H)-yl)aurate(1-)
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66% inhibition at 0.02 mM
protoporphyrin IX
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RLLGAP-(cis-4-aminocyclohexane carboxylic acid)-QLVSGI
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RLLGAP-(cis-4-aminocyclohexane carboxylic acid)-QLVSGI-betaAla-betaAla-PW
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RLLGAP-gamma-aminobutyric acid-QLVSGI
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RLLGAP-gamma-aminobutyric acid-QLVSGI-betaAla-betaAla-PW
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RLLGAPV-(cis-4-aminocyclohexane carboxylic acid)-RQLVSGI
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63% inhibition at 0.005 mM
RLLGAPV-(cis-4-aminocyclohexane carboxylic acid)-RQLVSGI-betaAla-betaAla-PW
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RLLGAPV-gamma-aminobutyric acid-RQLVSGI
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57% inhibition at 0.005 mM
RLLGAPV-gamma-aminobutyric acid-RQLVSGI-betaAla-betaAla-PW
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[(3R)-2-oxo-1-(2-oxo-2-[[(2E)-4-oxopent-2-en-1-yl]amino]ethyl)-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-3-yl]methyl [4-chloro-2-(trifluoromethyl)phenyl]carbamate
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[(5Z)-2,4-dioxo-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidin-3-yl]acetic acid
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[3-benzyl-2-(pyridin-2-yl-kappaN)-4,5-dihydro-1,3-oxazol-3-ium-kappaN](dichloro)aurate(1-) hexafluorophosphate
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54% inhibition at 0.02 mM
additional information
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not inhibited by pyrimethamine
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2440
Abz-KLRFSKQ-EDDnp
Plasmodium falciparum
-
in 0.1 M sodium acetate, pH 5.5, at 37°C
202548
1850
Abz-KLRSSKQ-EDDnp
Plasmodium falciparum
-
in 0.1 M sodium acetate, pH 5.5, at 37°C
10357
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
2-[3-(isobutylamino-methyl)-2-oxo-5-phenyl-2,3-dihydrobenzo[e][1,4]diazepin-1-yl]-N-(4-oxo-pent-2-enyl)-acetamide
-
Ki above 0.1 mM, in 50 mM sodium acetate buffer, pH 5.5 containing 10 mM dithiothreitol, at 22°C
0.000065
benzyl [1-[(2-oxoethyl)carbamoyl]cyclohexyl]carbamate
pH and temperature not specified in the publication
0.0012
benzyl [1-[(cyanomethyl)carbamoyl]cyclohexyl]carbamate
pH and temperature not specified in the publication
0.0000065
chicken egg white cystatin
-
in 100 mM NaOAc, 10 mM dithiothreitol, at 22°C, pH not specified in the publication
-
0.00029
E-64
0.0021
N-(cyanomethyl)-1-([[(1S,2S)-2-phenylcyclopropyl]carbonyl]amino)cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0082
N-(cyanomethyl)-1-[(3-cyclopentylpropanoyl)amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0076
N-(cyanomethyl)-1-[(cyclopentylacetyl)amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0073
N-(cyanomethyl)-1-[[3-(2,3-dimethoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0052
N-(cyanomethyl)-1-[[3-(2-methoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0089
N-(cyanomethyl)-1-[[3-(4-methoxyphenyl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0172
N-(cyanomethyl)-1-[[3-(furan-2-yl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0094
N-(cyanomethyl)-1-[[3-(pyridin-3-yl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.0088
N-(cyanomethyl)-1-[[3-(thiophen-2-yl)propanoyl]amino]cyclohexanecarboxamide
pH and temperature not specified in the publication
0.002
RLLGAPV-(cis-4-aminocyclohexane carboxylic acid)-RQLVSGI
-
in 100 mM NaOAc, 10 mM dithiothreitol, at 22°C, pH not specified in the publication
0.0025
RLLGAPV-(cis-4-aminocyclohexane carboxylic acid)-RQLVSGI-betaAla-betaAla-PW
-
in 100 mM NaOAc, 10 mM dithiothreitol, at 22°C, pH not specified in the publication
0.0025
RLLGAPV-gamma-aminobutyric acid-RQLVSGI
-
in 100 mM NaOAc, 10 mM dithiothreitol, at 22°C, pH not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02908
(5Z)-3-(2,4-difluorobenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
(5Z)-3-(3-methoxybenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
(5Z)-3-(4-fluorobenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.04335
(5Z)-3-(4-methylbenzyl)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.03624
(5Z)-3-benzyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
(5Z)-3-ethyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
(5Z)-3-methyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
(5Z)-3-[4-(trifluoromethyl)benzyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.04784
(5Z)-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01216
(S)-2-(1-(4-benzylpiperidin-1-yl)-1-oxo-3-phenylpropan-2-yl)isoindoline-1,3-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.04824
(S)-2-(1-(4-benzylpiperidin-1-yl)-3-methyl-1-oxobutan-2-yl)isoindoline-1,3-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.04423
(S)-2-(1-(4-benzylpiperidin-1-yl)-4-methyl-1-oxopentan-2-yl)isoindoline-1,3-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.0527
2,2'-((2S,2'S)-piperazine-1,4-diylbis(1-oxo-3-phenylpropane-2,1-diyl))bis(isoindoline-1,3-dione)
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.01689
2,2'-((2S,2'S)-piperazine-1,4-diylbis(3-methyl-1-oxobutane-2,1-diyl))bis(isoindoline-1,3-dione)
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.203
2,2'-((2S,2'S)-piperazine-1,4-diylbis(4-methyl-1-oxopentane-2,1-diyl))bis(isoindoline-1,3-dione)
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.00597
2,2'-((2S,2'S,3R,3'R)-piperazine-1,4-diylbis(3-methyl-1-oxopentane-2,1-diyl))bis(isoindoline-1,3-dione)
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.05379
2-((2S,3S)-1-(4-benzylpiperidin-1-yl)-3-methyl-1-oxopentan-2-yl)isoindoline-1,3-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.04943
2-[(2S)-1-(morpholin-4-yl)-1-oxo-3-phenylpropan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.03605
2-[(2S)-1-oxo-3-phenyl-1-(piperidin-1-yl)propan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.0876
2-[(2S)-1-oxo-3-phenyl-1-(pyrrolidin-1-yl)propan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.2056
2-[(2S)-3-methyl-1-(morpholin-4-yl)-1-oxobutan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.1305
2-[(2S)-3-methyl-1-oxo-1-(piperidin-1-yl)butan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.1416
2-[(2S)-3-methyl-1-oxo-1-(pyrrolidin-1-yl)butan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.1393
2-[(2S)-4-methyl-1-(morpholin-4-yl)-1-oxopentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.2179
2-[(2S)-4-methyl-1-oxo-1-(piperidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.2362
2-[(2S)-4-methyl-1-oxo-1-(pyrrolidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.06057
2-[(2S,3R)-3-methyl-1-(morpholin-4-yl)-1-oxopentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.1067
2-[(2S,3R)-3-methyl-1-oxo-1-(piperidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.1464
2-[(2S,3R)-3-methyl-1-oxo-1-(pyrrolidin-1-yl)pentan-2-yl]-1H-isoindole-1,3(2H)-dione
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.02908
3-adamantyl-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01264
3-[2-oxo-2-(4-(2-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01625
3-[2-oxo-2-(4-(3-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01123
3-[2-oxo-2-(4-(4-chlorophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01224
3-[2-oxo-2-(4-(4-cyanophenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-(4-methoxyphenyl)piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-cyclohexylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-methylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.03375
3-[2-oxo-2-(4-phenoxypiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
-
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4-dichlorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,4-dimethoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.03528
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2,5-ditrifluoromethylbenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
-
0.02388
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-bromobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.0158
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-chlorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.03528
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(2-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
-
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3,4-difluorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-carboxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.03325
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-fluorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.02389
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-isopropylbenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-methoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.04533
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-methylbenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.04497
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(3-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-chlorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-fluorobenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-methoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.04571
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-methylbenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.01344
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-trifluoromethoxybenzylidene)1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
-
0.01804
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-(4-trifluoromethylbenzylidene)
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.0388
3-[2-oxo-2-(4-phenylpiperazin-1-yl)ethyl]-5-benzylidene-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(4-pyridylpiperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.05
3-[2-oxo-2-(piperazin-1-yl)ethyl]-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidine-2,4-dione
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
0.0027
3-[4-[(2-phenylethyl)sulfamoyl]phenyl]-N-(5-phenyl-1,3,4-thiadiazol-2-yl)propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.0132
3-[4-[(3-chloro-4-fluorophenyl)sulfamoyl]phenyl]-N-(2,3-dihydro-1,4-benzodioxin-6-yl)propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.0098
3-[4-[(3-chloro-4-fluorophenyl)sulfamoyl]phenyl]-N-(5-ethyl-1,3,4-thiadiazol-2-yl)propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.000159
artemisinin
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.003
bilirubin
Plasmodium falciparum
-
at pH 5.5 and 37°C
0.000066
Chloroquine diphosphate
Plasmodium falciparum
-
in 100 mM acetate buffer pH 5.5, at 37°C
0.0009
coproporphyrin
Plasmodium falciparum
-
at pH 5.5 and 37°C
0.002
deuteroporphyrin 3,7,12,17-tetramethyl-21H,23H-porphyrin-2,18-dipropionic acid dihydrochloride
Plasmodium falciparum
-
at pH 5.5 and 37°C
-
0.00048
deuteroporphyrin IX 2,4 (4,2) hydroxyvinyl
Plasmodium falciparum
-
at pH 5.5 and 37°C
-
0.00062
deuteroporphyrin IX 2-vinyl, 4-hydroxymethyl
Plasmodium falciparum
-
at pH 5.5 and 37°C
-
0.000017 - 0.000047
E-64
0.000515
heme
Plasmodium falciparum
-
at pH 5.5 and 37°C
0.002
isohematoporphyrin IX
Plasmodium falciparum
-
at pH 5.5 and 37°C
-
0.005
mesoporphyrin IX dimethyl ester
Plasmodium falciparum
-
IC50 above 0.005 mM, at pH 5.5 and 37°C
-
0.0089
N-(1,3-benzothiazol-2-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.0542
N-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.0081
N-(5-ethyl-1,3,4-thiadiazol-2-yl)-3-[4-[(2-phenylethyl)sulfamoyl]phenyl]propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.007
N-(5-ethyl-1,3,4-thiadiazol-2-yl)-3-[4-[(4-fluorophenyl)sulfamoyl]phenyl]propanamide
Plasmodium falciparum
-
in 100 mM sodium acetate, pH 5.5, 10 mM dithiothreitol, at 22°C
0.005
N-methyl mesoporphyrin
Plasmodium falciparum
-
IC50 above 0.005 mM, at pH 5.5 and 37°C
0.00037
N-methyl protoporphyrin IX
Plasmodium falciparum
-
at pH 5.5 and 37°C
0.00055
protoporphyrin IX
Plasmodium falciparum
-
at pH 5.5 and 37°C
0.05
[(5Z)-2,4-dioxo-5-(2,4,6-trimethoxybenzylidene)-1,3-thiazolidin-3-yl]acetic acid
Plasmodium falciparum
-
IC50 above 0.05 mM, in 100 mM sodium acetate, pH 5.5, at 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
1 * 37000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 37000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with the inhibitory C-terminal domain of inhibitors of cysteine protease from Plasmodium berghei, sitting drop vapor diffusion method, using 200 mM sodium acetate, 27.5 mM CdCl2, 100 mM MES (pH 5.0)
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography, HiTrap Q anion-exchange column chromatography, and Superdex 75 gel filtration
-
nickel affinity column chromatography and Q Sepharose column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli Origami(DE3) cells
-
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE