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Literature summary for 3.4.22.B69 extracted from

  • Cotrin, S.S.; Gouvea, I.E.; Melo, P.M.; Bagnaresi, P.; Assis, D.M.; Araujo, M.S.; Juliano, M.A.; Gazarini, M.L.; Rosenthal, P.J.; Juliano, L.; Carmona, A.K.
    Substrate specificity studies of the cysteine peptidases falcipain-2 and falcipain-3 from Plasmodium falciparum and demonstration of their kininogenase activity (2013), Mol. Biochem. Parasitol., 187, 111-116.
    View publication on PubMed
Search for more literature for 3.4.22.B69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
high molecular weight kininogen + H2O Plasmodium falciparum
-
 Met-Lys-bradykinin + Lys-bradykinin + bradykinin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-KLRFSKQ-EDDnp + H2O
-
 Plasmodium falciparum ?
-
 ?
Abz-KLRSSKQ-EDDnp + H2O
-
 Plasmodium falciparum ?
-
 ?
Abz-KLRSXKQ-EDDnp + H2O
-
 Plasmodium falciparum Abz-KLRS + XKQ-EDDnp
-
 ?
Abz-KLRXSKQ-EDDnp + H2O
-
 Plasmodium falciparum Abz-KLR + XSKQ-EDDnp
-
 ?
Abz-KLXSSKQ-EDDnp + H2O
-
 Plasmodium falciparum Abz-KLX + SSKQ-EDDnp
-
 ?
Abz-KXRSSKQ-EDDnp + H2O
-
 Plasmodium falciparum Abz-KXR + SSKQ-EDDnp
-
 ?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O the peptide corresponds to the Met375 to Ile393 sequence of high molecular weight kininogen. The enzyme preferentially accommodates at the S1 subsite the positively charged residue Arg, followed by Gln. At the P2 position, the enzyme shows a clear preference for the hydrophobic aliphatic residue Leu over Phe. The subsite S3 of FP-2 shows a broad specificity with slight preference of Lys in the P3 position Plasmodium falciparum ? the generated fragments are KRPPGFSPFR (Lys-bradykinin, 63%), RPPGFSPFR (bradykinin, 30%), and MKRPPGFSPFR (Met-Lys-bradykinin, 7%) ?
Abz-XLRSSKQ-EDDnp + H2O
-
 Plasmodium falciparum Abz-XLR + SSKQ-EDDnp
-
 ?
high molecular weight kininogen + H2O
-
 Plasmodium falciparum Met-Lys-bradykinin + Lys-bradykinin + bradykinin + ?
-
 ?

Synonyms

Synonyms Comment Organism
FP-2
-
 Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1850
-
 Abz-KLRSSKQ-EDDnp in 0.1 M sodium acetate, pH 5.5, at 37°C Plasmodium falciparum
2440
-
 Abz-KLRFSKQ-EDDnp in 0.1 M sodium acetate, pH 5.5, at 37°C Plasmodium falciparum