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Information on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end) and Organism(s) Phanerodontia chrysosporium and UniProt Accession Q7LIJ0

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Phanerodontia chrysosporium
UNIPROT: Q7LIJ0 not found.
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The taxonomic range for the selected organisms is: Phanerodontia chrysosporium
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
glucanase, cbh i, cbh ii, cellobiohydrolase ii, celluclast 1.5, cellobiosidase, cellobiohydrolase 1, cel7d, avicelase ii, cbh9a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-beta-cellobiohydrolase
-
-
-
-
1,4-beta-D-glucan cellobiohydrolase
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-
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1,4-beta-glucan cellobiohydrolase
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-
-
-
1,4-beta-glucan cellobiosidase
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-
-
-
avicelase
-
-
-
-
avicelase II
-
-
-
-
beta-1,4-glucan cellobiohydrolase
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-
-
-
beta-1,4-glucan cellobiosylhydrolase
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-
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-
Beta-1,4-glycanase CEX
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-
-
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Beta-glucancellobiohydrolase
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-
-
-
C1 cellulase
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-
-
-
CBH 1
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-
-
-
CBH II
CBHI
-
-
-
-
CBHII
-
-
-
-
CBP120
-
-
-
-
CBP95
-
-
-
-
Cel6A
-
-
-
-
cellobiohydrolase
cellobiohydrolase class II
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-
cellobiohydrolase I
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-
-
-
cellobiohydrolase II
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-
-
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cellobiohydrolase, exo-
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-
cellobiosidase
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-
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-
cellobiosidase, 1,4-beta-glucan
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-
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cellulase, C1
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-
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-
exo-beta-1,4-glucan cellobiohydrolase
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-
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exo-cellobiohydrolase
-
-
-
-
exocellobiohydrolase
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-
-
-
exoglucanase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan cellobiohydrolase (non-reducing end)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37329-65-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-D-cellobioside + H2O
4-methylumbelliferol + beta-D-cellobiose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl beta-D-lactoside + H2O
4-methylumbelliferol + beta-D-lactose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-lactoside + H2O
4-nitrophenol + beta-lactose
show the reaction diagram
-
-
-
?
avicel + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
?
cellulose + H2O
cellobiose + ?
show the reaction diagram
the enzyme hydrolyzes beta-1,4-linkages
-
-
?
cellulose + H2O
cellobiose + D-glucose + cellotriose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
p-nitrophenol + cellobiose
show the reaction diagram
-
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cellobiose
-
presence of cellobiose dehydrogenase relieves inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.384
p-nitrophenyl-beta-D-cellobioside
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.6
purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-lactoside
2.15
purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-cellobioside
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q7LIJ0_PHACH
510
0
53793
TrEMBL
Mitochondrion (Reliability: 4)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 50000-60000, recombinant enzyme, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C313S
-
random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
5 min, loss of 50% of activity of the purified recombinant enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cbhl.2, expression in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies
gene cbhl.2, genomic library construction, DNA and amino acid sequence determination and analysis, expression of inactive enzyme in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
refolding of recombinant enzyme, after solubilization and denaturation by 8 M urea from Escherichia coli inclusion bodies, by dialysis of enzyme in 10 mM DTT, PMSF, 2-mercaptoethanol, at 4°C against 1 l of 50 mM Tris, pH 8.0, containing 5 mM CaCl2, for 2 days with 3times of buffer change
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Igarashi, K.; Samejima, M.; Eriksson, K.E.L.
Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition
Eur. J. Biochem.
253
101-106
1998
Phanerodontia chrysosporium, Phanerodontia chrysosporium CBH I
Manually annotated by BRENDA team
Munoz, I.G.; Ubhayasekera, W.; Henriksson, H.; Szabo, I.; Pettersson, G.; Johansson, G.; Mowbray, S.L.; Stahlberg, J.
Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes
J. Mol. Biol.
314
1097-1111
2001
Phanerodontia chrysosporium
Manually annotated by BRENDA team
Howard, R.L.
Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhl.2)
Afr. J. Biotechnol.
4
1180-1184
2005
Phanerodontia chrysosporium (Q7LHI2)
-
Manually annotated by BRENDA team
Howard, R.L.
Refolding and characterization of a heterologous expressed Phanerochaete chrysosporium cellobiohydrolase (CBHl.2)
Afr. J. Biotechnol.
4
1185-1188
2005
Phanerodontia chrysosporium (Q7LHI2)
-
Manually annotated by BRENDA team
Heinzelman, P.; Snow, C.D.; Smith, M.A.; Yu, X.; Kannan, A.; Boulware, K.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability
J. Biol. Chem.
284
26229-26233
2009
Phanerodontia chrysosporium, Trichoderma reesei (P07987), Humicola insolens (Q9C1S9), Humicola insolens
Manually annotated by BRENDA team