Cloned (Comment) | Organism |
---|---|
gene cbhl.2, expression in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q7LHI2 | i.e. Sporotrichum pruinosum, strain ME446, gene cbhl.2 | - |
Renatured (Comment) | Organism |
---|---|
refolding of recombinant enzyme, after solubilization and denaturation by 8 M urea from Escherichia coli inclusion bodies, by dialysis of enzyme in 10 mM DTT, PMSF, 2-mercaptoethanol, at 4°C against 1 l of 50 mM Tris, pH 8.0, containing 5 mM CaCl2, for 2 days with 3times of buffer change | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-lactoside | Phanerodontia chrysosporium |
2.15 | - |
purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-cellobioside | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Phanerodontia chrysosporium | 4-methylumbelliferol + beta-D-cellobiose | - |
? | |
4-methylumbelliferyl beta-D-lactoside + H2O | - |
Phanerodontia chrysosporium | 4-methylumbelliferol + beta-D-lactose | - |
? | |
4-nitrophenyl beta-cellobioside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-cellobiose | - |
? | |
4-nitrophenyl beta-lactoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-lactose | - |
? | |
avicel + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
cellulose + H2O | the enzyme hydrolyzes beta-1,4-linkages | Phanerodontia chrysosporium | cellobiose + ? | - |
? | |
additional information | substrate specificity, overview | Phanerodontia chrysosporium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000-60000, recombinant enzyme, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
Cbhl.2 | - |
Phanerodontia chrysosporium |
cellobiohydrolase | - |
Phanerodontia chrysosporium |
Cellulase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Phanerodontia chrysosporium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
5 min, loss of 50% of activity of the purified recombinant enzyme | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Phanerodontia chrysosporium |