Information on EC 3.2.1.86 - 6-phospho-beta-glucosidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.2.1.86
-
RECOMMENDED NAME
GeneOntology No.
6-phospho-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycolysis / Gluconeogenesis
-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-glucosyl-(1->4)-D-glucose glucohydrolase
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-phospho-alpha-glucosidase
Q9AI65
-
6-phospho-alpha-glucosidase
Erwinia rhapontici DSM448
Q9AI65
-
-
6-phospho-beta-glucosidase
Q6MSD6
-
6-phospho-beta-glucosidase
-
-
bglD
-
gene name
P-beta-glc
Lactobacillus acidophilus JCM1132T
-
-
-
P-beta-glc
Lactobacillus johnsonii JCM2122
-
-
-
P-beta-glc
-
-
PalH
Q9AI65
-
PalH
Erwinia rhapontici DSM448
Q9AI65
-
-
phospho-alpha-glucosidase
Q9AI65
-
phospho-alpha-glucosidase
Erwinia rhapontici DSM448
Q9AI65
-
-
phospho-beta-glucosidase
-
-
-
-
phospho-beta-glucosidase
-
-
phospho-beta-glucosidase
Lactobacillus acidophilus JCM1132T
-
-
-
phospho-beta-glucosidase
Lactobacillus johnsonii JCM2122
-
-
-
phospho-beta-glucosidase
-
-
phospho-beta-glucosidase
-
-
phospho-beta-glucosidase A
-
-
-
-
phosphocellobiase
-
-
-
-
Cellobiose-6-phosphate hydrolase
-
-
-
-
additional information
Q9AI65
the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site
additional information
Erwinia rhapontici DSM448
Q9AI65
the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site
-
CAS REGISTRY NUMBER
COMMENTARY
37205-51-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain DSM448, gene palH
UniProt
Manually annotated by BRENDA team
Erwinia rhapontici DSM448
strain DSM448, gene palH
UniProt
Manually annotated by BRENDA team
strain JCM1132T and LA117
-
-
Manually annotated by BRENDA team
Lactobacillus acidophilus JCM1132T
strain JCM1132T and LA117
-
-
Manually annotated by BRENDA team
strain MEP181R45, strain MEP181R50 and strain MEP181R51
-
-
Manually annotated by BRENDA team
strain JCM1134T, strain MEP181R52, strain MEP181R53, strain MEP181R54 and strain NIAI C-9
-
-
Manually annotated by BRENDA team
strain MEP181R55 and strain MEP181R56
-
-
Manually annotated by BRENDA team
subsp. lactis, DSM 7290
-
-
Manually annotated by BRENDA team
very low activity in strain MEP181R57 and MEP181R58
-
-
Manually annotated by BRENDA team
strain MEP181R65, strain ATCC33323T, strain JCM1031, strain LA2, strain OLL2836, strain MEP181R34, strain MEP181R35, strain MEP181R36, strain MEP181R40, strain MEP181R41 (high activity), strain MEP181R42 (high activity), strain MEP181R43 (high activity), strain MEP181R44 (high activity), strain MEP181R46 (high activity), strain MEP181R48 (high activity), strain MEP181R49 (high activity), strain OLL 2948 and strain MEP181R46 show the highest activity
-
-
Manually annotated by BRENDA team
strain JCM1017 and strain JCM2122
-
-
Manually annotated by BRENDA team
strain OLL2848, strain MEP181R67 (very low activity), strain MEP181R68 (low activity), strain MEP181R70 (low activity), strain MEP181R72 (very low activity), strain MEP181R74, strain MEP181R75 (very low activity)
-
-
Manually annotated by BRENDA team
subsp. mycoides SC
SwissProt
Manually annotated by BRENDA team
subsp. carotovorum LY34
-
-
Manually annotated by BRENDA team
recombinant
Uniprot
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
high activity
-
-
?
2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,5-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
2-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
3,4-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
3,5-dichlorophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
3-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-chloro-2-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
4-cyanophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
4-methylumbelliferone + beta-D-glucopyranoside 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
Q6MSD6
strains expressing the Bgl isoform Val204 show a lower hydrolysing activity on 4-nitrophenyl-beta-D-glucopyranoside when compared to strains that possess the Bgl isoform Ala204
-
-
?
4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
4-tert-butylphenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
show the reaction diagram
P17411
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
cellobiitol monophosphate + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-, P17411
-
-
-
-
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Q9X108
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
-
esculin 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
gentiobiose monophosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-, P17411
-
-
-
-
gentiobiose monophosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose monophosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
show the reaction diagram
-
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
methyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
methyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
P17411
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactose + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
very low activity
-
-
?
p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O
p-nitrophenol + 6-phospho-beta-D-thioglucose
show the reaction diagram
Q9X108, -
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
P17411
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
Q9X108
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
-
isoenzyme B
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucopyranose 6-phosphate
show the reaction diagram
Q9X108, -
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucose + H2O
p-nitrophenol + D-glucose
show the reaction diagram
-
very low activity
-
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
show the reaction diagram
-, P17411
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
-
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
show the reaction diagram
-
isoenzyme B
-
-
?
sophorose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
methyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
-
expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization
-
-
-
additional information
?
-
Q6MSD6
single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia
-
-
-
additional information
?
-
-, Q9AI65
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
-
additional information
?
-
-
the enzyme is not active against non-phosphorylated beta-glucosides
-
-
-
additional information
?
-
Erwinia rhapontici DSM448
Q9AI65
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Q9X108
-
-
-
?
additional information
?
-
-
expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization
-
-
-
additional information
?
-
Q6MSD6
single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
-
enzyme is completely inactive in the absence of NAD+. Kd-value is 480 nM
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
Q9X108
much less effective activator than Mn2+
Ca2+
-
enhances activity, at 5 mM 11% increase in activity compared to activity without added ion
Mg2+
Q9X108
much less effective activator than Mn2+
Mg2+
-
enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion
Mn2+
Q9X108
best activator
Mn2+
-, Q9AI65
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Co2+
-
5 mM, 12% inhibition
Cu2+
-
5 mM, 8% inhibition
D-glucono-delta-lactone
-
-
DL-alpha-glycerol-phosphate
-
-
fructose-1,6-diphosphate
-
-
Hg2+
-
5 mM, complete inhibition
Mn2+
-
5 mM, 14% inhibition
phosphoenolpyruvate
-
-
Zn2+
-
5 mM, 13% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
Q9X108
-
dithiothreitol
Q9X108
-
NAD+
Q9X108
0.001 mM for highest activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0444
-
2,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0166
-
2,5-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0174
-
2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0314
-
3,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0414
-
3,5-dichlorophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0729
-
3-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.015
-
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0319
-
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0454
-
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0092
-
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
-
-
0.3
-
4-methylumbelliferyl-beta-D-glucopyransoide 6-phosphate
-, P17411
-
-
0.048
-
4-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0583
-
4-tert-butylphenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0977
-
arbutin 6-phosphate
-
-
0.23
0.5
arbutin 6-phosphate
-
-
0.31
-
arbutin 6-phosphate
-
-
0.35
-
arbutin 6-phosphate
-, P17411
-
0.23
0.5
cellobiitol monophosphate
-
-
0.25
-
cellobiitol monophosphate
-
-
0.1823
-
cellobiose 6-phosphate
-
-
0.23
0.5
cellobiose 6-phosphate
-
-
0.23
-
cellobiose 6-phosphate
-
-
1.3
-
cellobiose 6-phosphate
-, P17411
-
0.3355
-
gentiobiose 6-phosphate
-
-
0.23
0.5
gentiobiose monophosphate
-
-
-
12.5
-
gentiobiose monophosphate
-, P17411
-
-
0.24
-
gentiobiose phosphate
-
-
0.0875
-
laminaribiose 6-phosphate
-
-
0.19
-
methyl beta-D-glucoside 6-phosphate
-
isoenzyme B
0.28
-
methyl beta-D-glucoside 6-phosphate
-
isoenzyme A
2.22
-
methyl beta-D-glucoside 6-phosphate
-, P17411
-
0.23
0.5
methyl beta-D-glucoside monophosphate
-
-
0.27
-
methyl beta-D-glucoside phosphate
-
-
0.1384
-
methyl-beta-D-glucoside 6-phosphate
-
-
0.25
-
o-nitrophenyl beta-D-galactoside 6-phosphate
-
isoenzyme B
0.206
-
p-nitrophenyl 6-phospho-beta-D-thioglucoside
Q9X108, -
pH 7.5, 50C
0.21
-
p-nitrophenyl-beta-D-glucopyranoside
-
pH 7.0, 40C
0.44
-
p-nitrophenyl-beta-D-glucopyranoside 6-P
-, P17411
-
0.0291
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
0.041
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
Q9X108
pH 7.5
0.041
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
Q9X108, -
pH 7.5, 50C
0.18
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
isoenzyme B
0.33
-
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate
-
isoenzyme A
-
0.11
-
p-nitrothiophenyl beta-D-glucoside 6-phosphate
-
isoenzyme B
-
0.23
0.5
phenyl beta-D-glucoside 6-phosphate
-
-
0.46
-
phenyl beta-D-glucoside 6-phosphate
-
isoenzyme B
0.5
-
phenyl beta-D-glucoside 6-phosphate
-
-
0.6
-
phenyl beta-D-glucoside 6-phosphate
-
isoenzyme A
0.0886
-
Salicin 6-phosphate
-
-
0.23
0.5
Salicin 6-phosphate
-
-
0.44
-
Salicin 6-phosphate
-, P17411
-
0.5
-
Salicin 6-phosphate
-
-
0.51
-
Salicin 6-phosphate
-
isoenzyme B
0.1212
-
sophorose 6-phosphate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.29
-
2,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.95
-
2,5-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.21
-
2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.15
-
3,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
2.33
-
3,5-dichlorophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
4.69
-
3-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.69
-
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.79
-
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.01
-
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.99
-
4-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.21
-
4-tert-butylphenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
1.6
-
p-nitrophenyl 6-phospho-beta-D-thioglucoside
Q9X108, -
pH 7.5, 50C
1.9
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
Q9X108
pH 7.5
1.9
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
Q9X108, -
pH 7.5, 50C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.3
-
-
isoenzyme A, p-nitrophenyl beta-D-glucospyranoside
7
8
-
cellobiose 6-phosphate
7
9
-
isoenzyme B, p-nitrophenyl beta-D-glucospyranoside
7.5
-
-, Q9AI65
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-, Q9AI65
assay at
34
38
-, P17411
-
40
-
-
pH 7.0, hydrolysis of p-nitrophenyl-beta-D-glucopyranoside
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
50
-
20C: 55% of maximal activity, 50C: 48% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Erwinia rhapontici DSM448
-
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain K12)
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21000
-
-, P17411
gel filtration
50000
-
-, P17411
SDS-PAGE
52000
-
-
sucrose density gradient centrifugation
53000
-
-
SDS-PAGE
53560
-
-
MALDI-MS
54000
-
-
gel filtration chromatography
65000
-
-
isoenzyme B, SDS-PAGE
68000
-
-
isoenzyme A, SDS-PAGE
132000
-
-
isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement
142000
-
-
isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 53000, SDS-PAGE
?
-
x * 55000, SDS-PAGE
dimer
-
homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE
tetramer
-, P17411
4 * 50000
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging-drop vapor-diffusion method, crystal structure of the native selenomethionine-substituted enzyme (2.85 A resolution) and of the enzyme in complex with NAD+, Mn2+ and glucose 6-phosphate (2.55 A resolution)
Q9X108, -
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
10
-
for more than 1 h
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
28
-
isoenzyme B
25
45
-
isoenzyme A
60
-
-
10 min, 39% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, 2 months, purified enzyme, 60% loss of activity
-
isoenzyme A, 4C, 1 year, sterile starting buffer, 0% loss in activity
-
isoenzyme B, -20C, 2 months, sterile starting buffer, 50% loss of activity
-
-20C, 6 months, no significant decline in activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration
-, Q9AI65
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli
-, Q9AI65
expressed in the beta-glucoside-negative strain Lactobacillus helveticus 3036(62), transformed strain is able to grow with arbutin, cellobiose, salicin and methyl-beta-glucoside as substates
-
expressed in Escherichia coli
-
expressed in Escherichia coli
Q9X108
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E173S
-, Q9AI65
site-directed mutagenesis
E173S/I174V
-, Q9AI65
site-directed mutagenesis
I174V
-, Q9AI65
site-directed mutagenesis
E173S
Erwinia rhapontici DSM448
-
site-directed mutagenesis
-
E173S/I174V
Erwinia rhapontici DSM448
-
site-directed mutagenesis
-
I174V
Erwinia rhapontici DSM448
-
site-directed mutagenesis
-
E182A
-
inactive mutant
E374A
-
inactive mutant