Information on EC 3.2.1.86 - 6-phospho-beta-glucosidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.86
-
RECOMMENDED NAME
GeneOntology No.
6-phospho-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chitobiose degradation
-
-
Glycolysis / Gluconeogenesis
-
-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-glucosyl-(1->4)-D-glucose glucohydrolase
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
CAS REGISTRY NUMBER
COMMENTARY hide
37205-51-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain DSM448, gene palH
UniProt
Manually annotated by BRENDA team
strain DSM448, gene palH
UniProt
Manually annotated by BRENDA team
strain JCM1132T and LA117
-
-
Manually annotated by BRENDA team
strain JCM1132T and LA117
-
-
Manually annotated by BRENDA team
strain MEP181R45, strain MEP181R50 and strain MEP181R51
-
-
Manually annotated by BRENDA team
strain JCM1134T, strain MEP181R52, strain MEP181R53, strain MEP181R54 and strain NIAI C-9
-
-
Manually annotated by BRENDA team
strain MEP181R55 and strain MEP181R56
-
-
Manually annotated by BRENDA team
subsp. lactis, DSM 7290
-
-
Manually annotated by BRENDA team
very low activity in strain MEP181R57 and MEP181R58
-
-
Manually annotated by BRENDA team
-
Q045H2, Q045X2
UniProt
Manually annotated by BRENDA team
strain JCM1017 and strain JCM2122
-
-
Manually annotated by BRENDA team
strain OLL2848, strain MEP181R67 (very low activity), strain MEP181R68 (low activity), strain MEP181R70 (low activity), strain MEP181R72 (very low activity), strain MEP181R74, strain MEP181R75 (very low activity)
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. mycoides SC
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. carotovorum LY34
-
-
Manually annotated by BRENDA team
gene SP_0578 or bglA-2
Q97S37
UniProt
Manually annotated by BRENDA team
gene Spy1599
UniProt
Manually annotated by BRENDA team
gene Spy1599
UniProt
Manually annotated by BRENDA team
collected from pulp wastewater from a pulp mill in China
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,5-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
2-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
show the reaction diagram
3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
3,4-dinitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
3,5-dichlorophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
3-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-chloro-2-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
4-cyanophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
4-methylumbelliferone + beta-D-glucopyranoside 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
strains expressing the Bgl isoform Val204 show a lower hydrolysing activity on 4-nitrophenyl-beta-D-glucopyranoside when compared to strains that possess the Bgl isoform Ala204
-
-
?
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranoside 6-phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
show the reaction diagram
4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
4-tert-butylphenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
show the reaction diagram
cellobiitol monophosphate + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose 6-phosphate + H2O
beta-D-glucose + glucose 6-phosphate
show the reaction diagram
Q97S37
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
esculin 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
gentiobiose monophosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
laminaribiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
show the reaction diagram
methyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
show the reaction diagram
o-nitrophenyl-beta-D-galactose + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
very low activity
-
-
?
p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O
p-nitrophenol + 6-phospho-beta-D-thioglucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucopyranose 6-phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
show the reaction diagram
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucose + H2O
p-nitrophenol + D-glucose
show the reaction diagram
-
very low activity
-
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
show the reaction diagram
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
show the reaction diagram
sophorose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose 6-phosphate + H2O
beta-D-glucose + glucose 6-phosphate
show the reaction diagram
Q97S37
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
enzyme is completely inactive in the absence of NAD+. Kd-value is 480 nM
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3',5'-AMP
-
-
Co2+
-
5 mM, 12% inhibition
D-glucono-delta-lactone
-
-
DL-alpha-glycerol-phosphate
-
-
fructose-1,6-diphosphate
-
-
Hg2+
-
5 mM, complete inhibition
Mn2+
-
5 mM, 14% inhibition
phosphoenolpyruvate
-
-
Zn2+
-
5 mM, 13% inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
dithiothreitol
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0444
2,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0166
2,5-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0174
2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.66 - 0.97
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.0314
3,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0414
3,5-dichlorophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0729
3-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.015
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0319 - 0.0454
4-cyanophenyl 6-phospho-beta-D-glucoside
0.0092
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
-
-
0.3
4-methylumbelliferyl-beta-D-glucopyransoide 6-phosphate
-
-
0.048
4-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.478 - 4.8
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.0583
4-tert-butylphenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50C
0.0977 - 0.5
arbutin 6-phosphate
0.23 - 0.5
cellobiitol monophosphate
0.1823 - 1.515
cellobiose 6-phosphate
0.3355
gentiobiose 6-phosphate
-
-
0.23 - 12.5
gentiobiose monophosphate
0.24
gentiobiose phosphate
-
-
0.0875
laminaribiose 6-phosphate
-
-
0.19 - 2.22
methyl beta-D-glucoside 6-phosphate
0.23 - 0.5
methyl beta-D-glucoside monophosphate
-
-
0.27
methyl beta-D-glucoside phosphate
-
-
0.1384
methyl-beta-D-glucoside 6-phosphate
-
-
0.25
o-nitrophenyl beta-D-galactoside 6-phosphate
-
isoenzyme B
0.206
p-nitrophenyl 6-phospho-beta-D-thioglucoside
pH 7.5, 50C
0.21
p-nitrophenyl-beta-D-glucopyranoside
-
pH 7.0, 40C
0.44
p-nitrophenyl-beta-D-glucopyranoside 6-P
-
0.0291 - 0.18
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.33
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate
-
isoenzyme A
-
0.11
p-nitrothiophenyl beta-D-glucoside 6-phosphate
-
isoenzyme B
-
0.23 - 0.6
phenyl beta-D-glucoside 6-phosphate
0.0886 - 0.51
Salicin 6-phosphate
0.1212
sophorose 6-phosphate
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.29
2,4-dinitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
1.95
2,5-dinitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
1.21
2-nitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
2 - 41
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
1.15
3,4-dinitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
2.33
3,5-dichlorophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
4.69
3-nitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
1.69
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
0.79 - 1.01
4-cyanophenyl 6-phospho-beta-D-glucoside
0.99
4-nitrophenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
0.243 - 195
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
1.21
4-tert-butylphenyl 6-phospho-beta-D-glucoside
Thermotoga maritima
-
pH 7.5, 50C
138 - 170
cellobiose 6-phosphate
1.6
p-nitrophenyl 6-phospho-beta-D-thioglucoside
Thermotoga maritima
Q9X108
pH 7.5, 50C
1.9
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
44 - 59
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.333 - 410
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
90 - 150
cellobiose 6-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
-
isoenzyme A, p-nitrophenyl beta-D-glucospyranoside
7 - 9
-
isoenzyme B, p-nitrophenyl beta-D-glucospyranoside
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
Q045H2, Q045X2
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 40
-
-
20 - 50
-
20C: 55% of maximal activity, 50C: 48% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
gel filtration
50000
SDS-PAGE
52000
-
sucrose density gradient centrifugation
53000
-
SDS-PAGE
53560
-
MALDI-MS
54000
-
gel filtration chromatography
65000
-
isoenzyme B, SDS-PAGE
68000
-
isoenzyme A, SDS-PAGE
132000
-
isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement
142000
-
isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE
tetramer
4 * 50000
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme in apoform or complexed with thiocellobiose 6-phosphate, sitting drop vapour diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 20 mM Tris-Cl, pH 8.0, 100 mM NaCl with 0.001 ml of reservoir solution containing 15% PEG 5000MME, and 0.1 M sodium citrate, pH 5.6, 16C, X-ray diffraction structure determination and analysis at 2.0-2.4 A resolution, molecular replacement method
Q97S37
crystals with C2, P2 twinned, and P2 not twinned structures, hanging-drop vapour diffusion method, mixing equal volumes of 20 mg/ml protein with 0.1 M Bis-Tris propane, pH 7.5, 0.2 M sodium fluoride or sodium bromide, 20% PEG 3350 at 19C, X-ray diffraction structure determination and analysis at 2.51-2.61 A resolution, three-dimensional structure analysis of the enzyme in a number of crystal forms complicated by unusual crystallographic twinning, the enzyme shows a classical (beta/alpha)8-barrel structure, molecular replacement method
hanging-drop vapor-diffusion method, crystal structure of the native selenomethionine-substituted enzyme (2.85 A resolution) and of the enzyme in complex with NAD+, Mn2+ and glucose 6-phosphate (2.55 A resolution)
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
for more than 1 h
136924
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 28
-
isoenzyme B
25 - 45
-
isoenzyme A
60
-
10 min, 39% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
SPy1599 is subject to degradation during both storage and crystallization experiments
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 6 months, no significant decline in activity
-
4C, 2 months, purified enzyme, 60% loss of activity
-
isoenzyme A, 4C, 1 year, sterile starting buffer, 0% loss in activity
-
isoenzyme B, -20C, 2 months, sterile starting buffer, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration; recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration
Q045H2, Q045X2
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
Q97S37
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nickel afinity and anion exchange and again nickel affinity chromatography, followed by ultrafiltration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned from metagenome, library construction, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain Rosetta (DE3)
expressed in Escherichia coli
expressed in the beta-glucoside-negative strain Lactobacillus helveticus 3036(62), transformed strain is able to grow with arbutin, cellobiose, salicin and methyl-beta-glucoside as substates
-
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli
gene Spy1599, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SP_0578 or bglA-2, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Q97S37
LacG1, phylogenetic analysis; LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363
Q045H2, Q045X2
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E173S
site-directed mutagenesis
E173S/I174V
site-directed mutagenesis
I174V
site-directed mutagenesis
E173S
-
site-directed mutagenesis
-
E173S/I174V
-
site-directed mutagenesis
-
I174V
-
site-directed mutagenesis
-
E182A
-
inactive mutant
E374A
-
inactive mutant
E171A
Q97S37
site-directed mutagenesis, inactive mutant
E171Q
Q97S37
site-directed mutagenesis, inactive mutant
E364A
Q97S37
site-directed mutagenesis, inactive mutant
E364Q
Q97S37
site-directed mutagenesis, inactive mutant
K430A
Q97S37
site-directed mutagenesis, inactive mutant
M423A
Q97S37
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
S424A
Q97S37
site-directed mutagenesis, inactive mutant
W338A
Q97S37
site-directed mutagenesis, inactive mutant
Y126A
Q97S37
site-directed mutagenesis, inactive mutant
Y126F
Q97S37
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y303A
Q97S37
site-directed mutagenesis, inactive mutant
Y303F
Q97S37
site-directed mutagenesis, inactive mutant
Y432F
Q97S37
site-directed mutagenesis, inactive mutant
E179A
site-directed mutagenesis, inactive mutant
E372A
site-directed mutagenesis, inactive mutant
K435L
site-directed mutagenesis, inactive mutant
S427A
site-directed mutagenesis, the mutant shows slightly decreased activity but substrate affinities compared to the wild-type enzyme
S427C
site-directed mutagenesis, the mutant shows increased activity but substrate affinities compared to the wild-type enzyme
S427D
site-directed mutagenesis, almost inactive mutant
S427E
site-directed mutagenesis, almost inactive mutant
W420A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
W420F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Y311F
site-directed mutagenesis, inactive mutant
Y437F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Show AA Sequence (3318 entries)
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