Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Erwinia rhapontici |
Cloned (Comment) | Organism |
---|---|
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli | Erwinia rhapontici |
Protein Variants | Comment | Organism |
---|---|---|
E173S | site-directed mutagenesis | Erwinia rhapontici |
E173S/I174V | site-directed mutagenesis | Erwinia rhapontici |
I174V | site-directed mutagenesis | Erwinia rhapontici |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Erwinia rhapontici | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Erwinia rhapontici |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erwinia rhapontici | Q9AI65 | strain DSM448, gene palH | - |
Erwinia rhapontici DSM 448 | Q9AI65 | strain DSM448, gene palH | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration | Erwinia rhapontici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici | ? | - |
? | |
additional information | an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism | Erwinia rhapontici DSM 448 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phospho-alpha-glucosidase | - |
Erwinia rhapontici |
More | the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site | Erwinia rhapontici |
PalH | - |
Erwinia rhapontici |
phospho-alpha-glucosidase | - |
Erwinia rhapontici |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Erwinia rhapontici |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Erwinia rhapontici |