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Literature summary for 3.2.1.86 extracted from

  • Hall, B.G.; Pikis, A.; Thompson, J.
    Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations (2009), Mol. Biol. Evol., 26, 2487-2497.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NAD+
-
Erwinia rhapontici

Cloned(Commentary)

Cloned (Comment) Organism
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli Erwinia rhapontici

Protein Variants

Protein Variants Comment Organism
E173S site-directed mutagenesis Erwinia rhapontici
E173S/I174V site-directed mutagenesis Erwinia rhapontici
I174V site-directed mutagenesis Erwinia rhapontici

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Erwinia rhapontici
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Erwinia rhapontici

Organism

Organism UniProt Comment Textmining
Erwinia rhapontici Q9AI65 strain DSM448, gene palH
-
Erwinia rhapontici DSM 448 Q9AI65 strain DSM448, gene palH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration Erwinia rhapontici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici ?
-
?
additional information an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism Erwinia rhapontici DSM 448 ?
-
?

Synonyms

Synonyms Comment Organism
6-phospho-alpha-glucosidase
-
Erwinia rhapontici
More the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site Erwinia rhapontici
PalH
-
Erwinia rhapontici
phospho-alpha-glucosidase
-
Erwinia rhapontici

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Erwinia rhapontici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Erwinia rhapontici