Literature summary for 3.2.1.86 extracted from

Yang, C.; Niu, Y.; Li, C.; Zhu, D.; Wang, W.; Liu, X.; Cheng, B.; Ma, C.; Xu, P.
Characterization of a novel metagenome-derived 6-phospho-beta-glucosidase from black liquor sediment (2013), Appl. Environ. Microbiol., 79, 2121-2127.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned from metagenome, library construction, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain Rosetta (DE3)	uncultured organism

Protein Variants

Protein Variants	Comment	Organism
E179A	site-directed mutagenesis, inactive mutant	uncultured organism
E372A	site-directed mutagenesis, inactive mutant	uncultured organism
K435L	site-directed mutagenesis, inactive mutant	uncultured organism
S427A	site-directed mutagenesis, the mutant shows slightly decreased activity but substrate affinities compared to the wild-type enzyme	uncultured organism
S427C	site-directed mutagenesis, the mutant shows increased activity but substrate affinities compared to the wild-type enzyme	uncultured organism
S427D	site-directed mutagenesis, almost inactive mutant	uncultured organism
S427E	site-directed mutagenesis, almost inactive mutant	uncultured organism
W420A	site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme	uncultured organism
W420F	site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme	uncultured organism
Y311F	site-directed mutagenesis, inactive mutant	uncultured organism
Y437F	site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme	uncultured organism

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	uncultured organism
Fe2+	-	uncultured organism
Fe3+	-	uncultured organism
Ni2+	-	uncultured organism

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.8	-	4-nitrophenyl-beta-D-glucopyranoside 6-phosphate	pH 7.0, 37°C	uncultured organism

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stabilizes the enzyme activity	uncultured organism
Mg2+	stabilizes the enzyme activity	uncultured organism
Mn2+	stabilizes the enzyme activity	uncultured organism

Organism

Organism	UniProt	Comment	Textmining
uncultured organism	L7Y2Z6	collected from pulp wastewater from a pulp mill in China	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nickel afinity and anion exchange and again nickel affinity chromatography, followed by ultrafiltration	uncultured organism

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O	-	uncultured organism	4-nitrophenol + beta-D-glucopyranose 6-phosphate	-	?
additional information	the enzyme shows no beta-glucosidase activity, EC 3.2.1.21	uncultured organism	?	-	?

Synonyms

Synonyms	Comment	Organism
Pbgl25-217	-	uncultured organism

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	uncultured organism

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	uncultured organism

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	uncultured organism
additional information	conserved residues S427, Lys435, and Tyr437 act as gatekeepers in a phosphate-binding loop and play important roles in phosphate recognition, homology structure modelling, overview	uncultured organism

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Release 2023.1 (January 2023)