Information on EC 3.2.1.100 - mannan 1,4-mannobiosidase

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The expected taxonomic range for this enzyme is: Aeromonas hydrophila

EC NUMBER
COMMENTARY hide
3.2.1.100
-
RECOMMENDED NAME
GeneOntology No.
mannan 1,4-mannobiosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-beta-D-mannosidic linkages in (1->4)-beta-D-mannans, to remove successive mannobiose residues from the non-reducing chain ends
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-(1,4)-mannan degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-mannan mannobiohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
81811-49-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain F-25, ssp. anaerogenes
-
-
Manually annotated by BRENDA team
strain F-25, ssp. anaerogenes
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
codium mannan + H2O
mannobiose
show the reaction diagram
coffee mannan + H2O
mannobiose
show the reaction diagram
-
-
sole product
?
mannobiosylmannitol + H2O
mannobiose + mannitol
show the reaction diagram
-
-
-
?
mannopentaose + H2O
mannobiose + mannose
show the reaction diagram
mannotetraose
mannohexaose
show the reaction diagram
-
transglycosylation
-
?
mannotetraose + H2O
mannobiose
show the reaction diagram
mannotetraosylmannitol + H2O
mannobiose + mannosylmannitol
show the reaction diagram
-
-
-
?
mannotriose + H2O
mannobiose + mannose
show the reaction diagram
mannotriose + mannose
mannopentaose
show the reaction diagram
-
transglycosylation
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
1 mM, complete inhibition
Cu2+
-
1 mM, complete inhibition
EDTA
-
1 mM, 30-50% inhibition
Fe3+
-
1 mM, 30-50% inhibition
Hg2+
-
1 mM, complete inhibition
p-chloromercuribenzoate
-
1 mM, 30-50% inhibition
Pb2+
-
1 mM, 30-50% inhibition
Zn2+
-
1 mM, 30-50% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51
mannobiose
-
-
0.13
Mannopentaose
-
-
0.24
Mannotetraose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
20°C, 20 h
135706
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
15 min, stable below
55
-
15 min, complete loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE