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Literature summary for 3.2.1.100 extracted from
- Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25 (1982), J. Biochem., 91, 1181-1186.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, complete inhibition | Aeromonas hydrophila |  |
| Cu2+ | 1 mM, complete inhibition | Aeromonas hydrophila | |
| EDTA | 1 mM, 30-50% inhibition | Aeromonas hydrophila | |
| Fe3+ | 1 mM, 30-50% inhibition | Aeromonas hydrophila | |
| Hg2+ | 1 mM, complete inhibition | Aeromonas hydrophila | |
| p-chloromercuribenzoate | 1 mM, 30-50% inhibition | Aeromonas hydrophila | |
| Pb2+ | 1 mM, 30-50% inhibition | Aeromonas hydrophila | |
| Zn2+ | 1 mM, 30-50% inhibition | Aeromonas hydrophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
Mannopentaose | - |
Aeromonas hydrophila | |
| 0.24 | - |
Mannotetraose | - |
Aeromonas hydrophila | |
| 0.51 | - |
mannobiose | - |
Aeromonas hydrophila | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aeromonas hydrophila | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
gel filtration | Aeromonas hydrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas hydrophila | - |
ssp. anaerogenes | - |
| Aeromonas hydrophila F-25 | - |
ssp. anaerogenes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| F-25 | Aeromonas hydrophila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Aeromonas hydrophila | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 86.5 | - |
- |
Aeromonas hydrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| codium mannan + H2O | - |
Aeromonas hydrophila | mannobiose | sole product | ? | |
| codium mannan + H2O | - |
Aeromonas hydrophila F-25 | mannobiose | sole product | ? | |
| coffee mannan + H2O | - |
Aeromonas hydrophila | mannobiose | sole product | ? | |
| mannobiosylmannitol + H2O | - |
Aeromonas hydrophila | mannobiose + mannitol | - |
? | |
| mannopentaose + H2O | - |
Aeromonas hydrophila | mannobiose + mannose | - |
? | |
| mannopentaose + H2O | - |
Aeromonas hydrophila F-25 | mannobiose + mannose | - |
? | |
| mannotetraose | transglycosylation | Aeromonas hydrophila | mannohexaose | - |
? | |
| mannotetraose + H2O | - |
Aeromonas hydrophila | mannobiose | - |
? | |
| mannotetraose + H2O | - |
Aeromonas hydrophila F-25 | mannobiose | - |
? | |
| mannotetraosylmannitol + H2O | - |
Aeromonas hydrophila | mannobiose + mannosylmannitol + ? | - |
? | |
| mannotriose + H2O | - |
Aeromonas hydrophila | mannobiose + mannose | - |
? | |
| mannotriose + H2O | - |
Aeromonas hydrophila F-25 | mannobiose + mannose | - |
? | |
| mannotriose + mannose | transglycosylation | Aeromonas hydrophila | mannopentaose | - |
? | |
| additional information | not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan | Aeromonas hydrophila | ? | - |
? | |
| additional information | not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan | Aeromonas hydrophila F-25 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aeromonas hydrophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
15 min, stable below | Aeromonas hydrophila |
| 55 | - |
15 min, complete loss of activity | Aeromonas hydrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aeromonas hydrophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | 20°C, 20 h | Aeromonas hydrophila |
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