Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21(DE3) | Xanthomonas citri |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method at 18°C | Xanthomonas citri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
beta-mannan | pH 5.5, 40°C | Xanthomonas citri | |
5.11 | - |
mannohexaose | pH 5.5, 40°C | Xanthomonas citri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99480 | - |
calculation from sequence | Xanthomonas citri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas citri | Q8PI23 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas citri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Xanthomonas citri | 4-nitrophenol + beta-D-mannopyranoside | - |
? | |
beta-mannan + H2O | - |
Xanthomonas citri | D-mannose + ? | - |
? | |
galactomannan + H2O | - |
Xanthomonas citri | D-mannose + ? | - |
? | |
mannobiose + H2O | increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides | Xanthomonas citri | mannose + | - |
? | |
mannohexaose + H2O | increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides | Xanthomonas citri | D-mannose + ? | - |
? | |
mannopentaose + H2O | increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides | Xanthomonas citri | D-mannose + ? | - |
? | |
mannotetraose + H2O | increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides | Xanthomonas citri | D-mannose + ? | - |
? | |
mannotriose + H2O | increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides | Xanthomonas citri | mannobiose + D-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-mannanase | - |
Xanthomonas citri |
XacMan2A | - |
Xanthomonas citri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Xanthomonas citri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 40% of maximal activity, 50°C: about 40% of maximal activity | Xanthomonas citri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
4 h, about 50% loss of activity | Xanthomonas citri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.53 | - |
beta-mannan | pH 5.5, 40°C | Xanthomonas citri | |
126.86 | - |
mannohexaose | pH 5.5, 40°C | Xanthomonas citri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Xanthomonas citri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 70% of maximal activity, pH 7.0: about 45% of maximal activity | Xanthomonas citri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.98 | - |
beta-mannan | pH 5.5, 40°C | Xanthomonas citri | |
24.82 | - |
mannohexaose | pH 5.5, 40°C | Xanthomonas citri |