Information on EC 3.1.4.16 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.1.4.16
-
RECOMMENDED NAME
GeneOntology No.
2',3'-cyclic-nucleotide 2'-phosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
hydrolysis of phosphoric ester
P06961
-
PATHWAY
KEGG Link
MetaCyc Link
Purine metabolism
-
Pyrimidine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside-2',3'-cyclic-phosphate 3'-nucleotidohydrolase
Also hydrolyses 3'-nucleoside monophosphates and bis-4-nitrophenyl phosphate, but not 3'-deoxynucleotides. Similar reactions are carried out by EC 3.1.27.3 (ribonuclease T1) and EC 3.1.27.5 (pancreatic ribonuclease).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2',3'-cAMP phosphodiesterase
-
-
2',3'-cyclic AMP 2'-phosphohydrolase
-
-
-
-
2',3'-cyclic AMP phosphodiesterase
-
-
-
-
2',3'-cyclic nucleoside monophosphate phosphodiesterase
-
-
-
-
2',3'-cyclic nucleotidase
-
-
-
-
2',3'-cyclic nucleotide 2'-phosphodiesterase
-
-
-
-
2',3'-cyclic nucleotide phosphohydrolase
-
-
-
-
2',3'-cyclic phosphodiesterase
-
-
-
-
2':3'-cyclic nucleotide phosphodiesterase:3'-nucleotidase
-
-
-
-
2':3'-cyclic phosphodiesterase
-
-
-
-
2,3-cyclic nucleotide phosphodiesterase
-
-
cyclic 2',3'-nucleotide phosphodiesterase
-
-
-
-
cyclic phosphodiesterase:3'-nucleotidase
-
-
-
-
cyclic-2',3'-nucleotide 2'-phosphohydrolase
-
-
-
-
phosphodiesterase, cyclic 2',3'-nucleotide 2'-
-
-
-
-
ribonucleoside 2',3'-cyclic phosphate diesterase
-
-
-
-
Rv0805
-
functions as a 3',5'-cyclic nucleotide phosphodiesterase but has also 2',3'-cyclic nucleotide phosphodiesterase function
YfcE-C74H
-
the mutant enzyme is strictly specific for 2',3'-cNMP, the C74H mutation uniquely confers a gain of function in hydrolysis of 2',3'-cAMP
CAS REGISTRY NUMBER
COMMENTARY
9037-18-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain A-40-2
-
-
Manually annotated by BRENDA team
Bacillus sp. A-40-2
strain A-40-2
-
-
Manually annotated by BRENDA team
activity towards 2',3'-cyclic nucleotides and 3',5'-cyclic nucleotides are associated with the same protein molecule
-
-
Manually annotated by BRENDA team
mutant deficient in the 2':3'-cyclic phosphodiesterase
-
-
Manually annotated by BRENDA team
CECT 2148
-
-
Manually annotated by BRENDA team
Fusarium culmorum CECT 2148
CECT 2148
-
-
Manually annotated by BRENDA team
strain 6746, activity towards 2',3'-cyclic nucleotides and 3',5'-cyclic nucleotides are associated with the same protein molecule
-
-
Manually annotated by BRENDA team
Lemna paucicostata 6746
strain 6746, activity towards 2',3'-cyclic nucleotides and 3',5'-cyclic nucleotides are associated with the same protein molecule
-
-
Manually annotated by BRENDA team
cv. Lukullus
-
-
Manually annotated by BRENDA team
O:8 strains WA-C and WA-314
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-cAMP + H2O
2'-AMP + 3'-AMP
show the reaction diagram
-, P06961
-
-
-
-
2',3'-cGMP + H2O
2'-GMP + 3'-GMP
show the reaction diagram
-, P06961
-
-
-
-
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-, P06961
-
-
-
-
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
Bacillus sp. A-40-2
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
Bacillus sp. A-40-2
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
Bacillus sp. A-40-2
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
Bacillus sp. A-40-2
-
-
-
-
?
5'-dTMP p-nitrophenyl ester
?
show the reaction diagram
-
-
-
?
5'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
weak activity
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
-, P06961
-
-
-
-
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-, P06961
-
-
-
-
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
P67095
-
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
CDP + H2O
CMP + phosphate
show the reaction diagram
-, P06961
-
-
-
-
CTP + H2O
CDP + phosphate
show the reaction diagram
-, P06961
-
-
-
-
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
-
r
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
P53052, -
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
formation of adenosine without accumulation of 3'-AMP
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
only product
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
the only major reaction product
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
-
Rv0805 is 150fold more active in hydrolyzing 2',3'-cAMP than 3',5'-cAMP, the enzyme hydrolyzes either P-O2' or P-O3' bonds to yield a mixture of 3'-AMP and 2'-AMP products, with a bias toward 3'-AMP
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
P67095
YfcE-C74H cleaves exclusively the P-O2' bond of 2',3'-cAMP
-
-
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
Fusarium culmorum CECT 2148
-
-
the only major reaction product
?
cyclic 2',3'-AMP + H2O
3'-AMP
show the reaction diagram
Lemna paucicostata 6746
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
P53052, -
-
-
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
only product
?
cyclic 2',3'-CMP + H2O
3'-CMP
show the reaction diagram
Fusarium culmorum, Fusarium culmorum CECT 2148
-
-
the only major reaction product
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
P67095
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
P53052, -
-
-
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
only product
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
Fusarium culmorum, Fusarium culmorum CECT 2148
-
-
the only major reaction product
?
cyclic 2',3'-GMP + H2O
3'-GMP
show the reaction diagram
Lemna paucicostata 6746
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
-
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
P53052, -
-
-
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
only product
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
Fusarium culmorum, Fusarium culmorum CECT 2148
-
-
the only major reaction product
?
cyclic 2',3'-UMP + H2O
3'-UMP
show the reaction diagram
Bacillus sp. A-40-2
-
-
-
-
?
cyclic 3',5'-AMP + H2O
5'-AMP
show the reaction diagram
P67095
weak activity
-
-
?
cyclic 3',5'-AMP + H2O
5'-AMP
show the reaction diagram
-
at 9.1% of the activity with 2',3'-cyclic AMP
-
-
?
cyclic 3',5'-AMP + H2O
5'-AMP
show the reaction diagram
-
at about 50% of the activity with 2',3'-AMP
-
?
cyclic 3',5'-AMP + H2O
5'-AMP
show the reaction diagram
-
weak activity, Rv0805 is 150fold more active in hydrolyzing 2',3'-cAMP than 3',5'-cAMP
-
-
?
cyclic 3',5'-AMP + H2O
5'-AMP
show the reaction diagram
Lemna paucicostata 6746
-
at about 50% of the activity with 2',3'-AMP
-
?
cyclic 3',5'-AMP + H2O
3'-AMP
show the reaction diagram
-
-
mixture of 3-AMP and 5-AMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate
?
cyclic 3',5'-CMP + H2O
3'-CMP
show the reaction diagram
-
-
mixture of 3-CMP and 5-CMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate
?
cyclic 3',5'-GMP + H2O
5'-GMP
show the reaction diagram
P67095
weak activity
-
-
?
cyclic 3',5'-GMP + H2O
5'-GMP
show the reaction diagram
-
weak activity
-
-
?
cyclic 3',5'-GMP + H2O
5'-GMP
show the reaction diagram
-
at 9.2% of the activity with 3',5'-cyclic AMP
-
?
cyclic 3',5'-GMP + H2O
5'-GMP
show the reaction diagram
Lemna paucicostata, Lemna paucicostata 6746
-
at about 50% of the activity with 2',3'-GMP
-
?
cyclic 3',5'-GMP + H2O
3'-GMP
show the reaction diagram
-
-
mixture of 3-GMP and 5-GMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate
?
cyclic 3',5'-UMP + H2O
3'-UMP
show the reaction diagram
-
-
mixture of 3-UMP and 5-UMP in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate
?
cyclic 3',5'-UMP + H2O
5'-UMP
show the reaction diagram
P67095
weak activity
-
-
?
cyclic 3',5'-UMP + H2O
5'-UMP
show the reaction diagram
-
weak activity
-
-
?
di(p-nitrophenyl) phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
P53052, -
-
-
?
di(p-nitrophenyl) phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
synthetic substrate
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
di-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
at 50% of the activity with cyclic 2',3'-UMP
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
-
?
NADP+ + H2O
NAD+ + phosphate
show the reaction diagram
-, P06961
-
-
-
-
nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
-
-
-
?
nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
P53052, -
-
-
?
nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
-
no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers
no further hydrolysis to nucleoside and phosphate
?
nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
-
RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2,3-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phospomonoesterases
-
?
nucleoside 3',5'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
-
no apparent base specificity, 2fold higher specific activity with nucleoside 2',3'-cyclic phosphate than with the 3',5'-cyclic isomers
mixture of nucleoside 3'-phosphate and nucleoside 5'-phosphate in a ratio of 6:1, no further hydrolysis to nucleoside and phosphate
?
p-nitrophenyl phenylphosphonate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
P67095
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-, P06961
-
-
-
-
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
weak activity
-
-
?
p-nitrophenyl phosphorylcholine + H2O
?
show the reaction diagram
P53052, -
-
-
?
thymidine p-nitrophenyl phosphate + H2O
thymidine + p-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-, P06961
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
higher activity towards 2',3'-cyclic nucleotides than towards 3',5'-cyclic nucleotides
-
-
-
additional information
?
-
-
3'-nucleotidase and 5'-nucleotidase activity resides in the same protein
-
-
-
additional information
?
-
-
3'-nucleotidase and 5'-nucleotidase activity resides in the same protein
-
-
-
additional information
?
-
-
3'-nucleotidase and 5'-nucleotidase activity resides in the same protein
-
-
-
additional information
?
-
-
3'-nucleotidase and 5'-nucleotidase activity resides in the same protein
-
-
-
additional information
?
-
P53052, -
bifunctional enzyme with 2,3-cyclic phosphodiesterase and 3-nucleotidase activity
-
?
additional information
?
-
-
not: 3,5-nucleoside monophosphates, nucleoside 5-, 3- or 2-monophosphates, tRNA, polyU
-
?
additional information
?
-
P53052, -
CpdB may be a fitness factor involved in the salvage of nucleotides from the environment
-
?
additional information
?
-
-
PdeB also shows high phosphomonoesterase activity against 5'-UMP, 3'-AMP, 3'-GMP, and low activity against 5'-dAMP
-
-
-
additional information
?
-
-
the enzyme does not have a preference for 2',3'-cAMP versus 2',3'-cGMP
-
-
-
additional information
?
-
-
the enzyme shows no activity toward 2'-AMP and 2'-GMP, 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeB
-
-
-
additional information
?
-
P67095
YfcE-C74H has no detectable ability to hydrolyze phosphomonoester substrates 5'-AMP, 3'-AMP, or 2'-AMP
-
-
-
additional information
?
-
Fusarium culmorum CECT 2148
-
not: 3,5-nucleoside monophosphates, nucleoside 5-, 3- or 2-monophosphates, tRNA, polyU
-
?
additional information
?
-
Lemna paucicostata 6746
-
higher activity towards 2',3'-cyclic nucleotides than towards 3',5'-cyclic nucleotides
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nucleoside 2',3'-cyclic phosphate + H2O
nucleoside 3'-phosphate
show the reaction diagram
-
RNA degradation, enzyme functions as an accessory ribonucleolytic activity that effectively hydrolyzes the primary products of RNase LE, a phosphate starvation-inducible ribonuclease generating 2,3-cyclic nucleoside monophosphates, to substrates for phosphate starvation-inducible phospomonoesterases
-
?
additional information
?
-
P53052, -
CpdB may be a fitness factor involved in the salvage of nucleotides from the environment
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
activates
Ca2+
-
stimulates cleavage of di-p-nitrophenyl phosphate
Ca2+
-
activates
Co2+
-
no activation
Co2+
-
10 mM, 2-3fold stimulation
Co2+
-
0.1-1 mM, 3-5fold activation
Co2+
-
no activation
Co2+
-
stimulates cleavage of dis-p-nitrophenyl phosphate
Co2+
-
activates
Co2+
-
PdeB is stimulated about 2fold by 0.05 mM Co2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate
KCN
-
slight activation of cyclic phosphodiesterase
Mg2+
-
activates
Mg2+
-
1-10 mM, slight increase of activity
Mg2+
P06961
activataes with 2'-AMP, KD: 0.24 +/- 0.1 mM; activates with 2',3'-cAMP, KD: 0.49 +/- 0.1 mM; activates with ATP, KD ~ 0.1 mM
Mn2+
-
10 mM, 2-3fold stimulation
Mn2+
-
0.2 mM, 6-10% stimulation
Mn2+
-
increases activity with bi-p-nitrophenyl phosphate, maximal activity at 10 mM
Mn2+
-
PdeB is stimulated about 2fold by 0.05 mM Mn2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate
Mn2+
P67095
dependent on
Mn2+
-
dependent on
Ni2+
P06961
activates with diphosphate, KD: 1.06 +/- 0.1 uM; activates with p-nitrophenyl phosphate, KD: 6.0 +/- 0.7 uM
Mo6+
-
activates
additional information
-
no requirement for metal ions
additional information
-
no requirement for metal ions
additional information
-
independent of divalent metal ions, e.g. Mg2+, Mn2+, Ca2+ and Co2+, all at 5mM
additional information
-
enzymatic activity is not stimulated by Ca2+, Mg2+, Fe2+, Fe3+, and Zn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5'-AMP
P06961
-
Cd2+
-
0.2 mM, 30% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 20% inhibition of 3'-AMP hydrolysis
Cd2+
-
0.1 mM, 59% inhibition
Co2+
-
0.1 mM, 80% inhibition
Cu2+
-
strong inhibition at micromolar concentrations, no effect on enzyme stability
Cu2+
-
5 mM, more than 90% inhibition
Hg2+
-
0.2 mM, 35% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 36% inhibition of 3'-AMP hydrolysis
MgCl2
-
maximal inhibition at 20 mM
MgSO4
-
slight inhibition
Na2SO4
-
slight inhibition
phosphate
-
PdeB is strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0)
phosphate
P67095
-
Zn2+
-
0.1 and 1 mM
Zn2+
-
0.2 mM, 90% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 78% inhibition of 3'-AMP hydrolysis
Zn2+
-
strong inhibition at micromolar concentrations, no effect on enzyme stability
Zn2+
-
5 mM, more than 90% inhibition
Zn2+
-
28% relative activity at 0.05 mM Zn2+, using cyclic 3',5'-AMP as substrate
Mn2+
-
0.5 mM, 50% inhibition
additional information
-
not inhibited by EDTA
-
additional information
-
not inhibited by 5 mM EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2'-AMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
3',5'-cAMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
3'-AMP
-
cleavage of 2',3'-cyclic nucleotides
5'-ADP
-
2',3'-cyclic nucleotide phosphodiesterase activity
5'-AMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
5'-ATP
-
2',3'-cyclic nucleotide phosphodiesterase activity
5'-CMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
5'-GMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
5'-UMP
-
2',3'-cyclic nucleotide phosphodiesterase activity
adenosine
-
2',3'-cyclic nucleotide phosphodiesterase activity
Br-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
Calmodulin
-
stimulates activity towards 2',3'-cyclic nucleotides and 3',5'-cyclic nucleotides
CH3COO-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
Cl-
-
activates 3'-nucleotidase activity
Cl-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
cytidine
-
2',3'-cyclic nucleotide phosphodiesterase activity
guanosine
-
2',3'-cyclic nucleotide phosphodiesterase activity
HCOO-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
NO3-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
uridine
-
2',3'-cyclic nucleotide phosphodiesterase activity
I-
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
additional information
-
monovalent cations activate in the order of decreasing effectiveness: Cl-, Br-/I-, NO3-, HCOO-, CH3COO-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.49
-
2',3'-cAMP
P06961
+/- 0.04
1.6
-
2',3'-cGMP
P06961
+/- 0.22
0.76
-
2'-AMP
P06961
+/- 0.13
0.022
-
3'-AMP
-
-
0.04
-
3'-AMP
-
-
0.045
-
3'-AMP
-
-
0.022
-
3'-UMP
-
-
0.053
-
3'-UMP
-
-
0.07
-
3'-UMP
-
-
0.19
-
ADP
P06961
+/- 0.02
0.18
-
ATP
P06961
+/- 0.01
0.022
-
bis(p-nitrophenyl)phosphate
-
-
0.094
-
bis(p-nitrophenyl)phosphate
-
-
0.28
-
bis(p-nitrophenyl)phosphate
-
-
0.7
-
bis(p-nitrophenyl)phosphate
-
-
1.2
-
bis(p-nitrophenyl)phosphate
-
-
0.9
-
bis-p-nitrophenyl phosphate
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
9.3
-
bis-p-nitrophenyl phosphate
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
0.53
-
CDP
P06961
+/- 0.09
0.13
-
CTP
P06961
+/- 0.01
0.0052
-
cyclic 2',3'-AMP
-
in 50 mM Tris-HCl, pH 8.0
0.3
-
cyclic 2',3'-AMP
-
pH 7.5, 30C
1.6
-
cyclic 2',3'-AMP
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
35
-
cyclic 2',3'-AMP
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
0.1
-
cyclic 2',3'-CMP
-
-
0.6
-
cyclic 2',3'-CMP
-
pH 7.5, 30C
0.1
-
cyclic 2',3'-GMP
-
pH 7.5, 30C
0.02
-
cyclic 2',3'-UMP
-
-
0.075
-
cyclic 2',3'-UMP
-
-
0.36
-
cyclic 2',3'-UMP
-
-
0.67
-
cyclic 2',3'-UMP
-
-
2
-
cyclic 2',3'-UMP
-
pH 7.5, 30C
0.2
-
di-p-nitrophenyl phosphate
-
with 200 mM NaCl
0.45
-
di-p-nitrophenyl phosphate
-
-
0.45
-
di-p-nitrophenyl phosphate
-
without NaCl
0.15
-
NADP
P06961
+/- 0.02
0.033
-
p-Nitrophenyl phenylphosphonate
-
-
1.7
-
p-nitrophenyl phosphate
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
6
-
p-nitrophenyl phosphate
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 5 mM MnCl2
6.2
-
p-nitrophenyl phosphate
P06961
+/- 0.46
0.167
-
thymidine p-nitrophenyl phosphate
-
-
0.1
-
diphosphate
P06961
+/- 0.004
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
7.63
-
2',3'-cAMP
P06961
+/- 0.48
1.97
-
2',3'-cGMP
P06961
+/- 0.08
3.09
-
2'-AMP
P06961
+/- 0.14
1.24
-
ADP
P06961
+/- 0.07
3.78
-
ATP
P06961
+/- 0.12
12.4
-
bis-p-nitrophenyl phosphate
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
72
-
bis-p-nitrophenyl phosphate
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
4.83
-
CDP
P06961
+/- 0.39
3.36
-
CTP
P06961
+/- 0.17
0.0006
-
cyclic 2',3'-AMP
-
in 50 mM Tris-HCl, pH 8.0
2.8
-
cyclic 2',3'-AMP
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
2.95
-
cyclic 2',3'-AMP
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
2.51
-
diphosphate
P06961
+/- 0.05
14.9
-
NADP
P06961
+/- 0.5
0.55
-
p-nitrophenyl phosphate
-
wild type enzyme, in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
3
-
p-nitrophenyl phosphate
P67095
in Tris-HCl buffer (pH 8.5) in the presence of 0.5 mM MnCl2
10.3
-
p-nitrophenyl phosphate
P06961
+/- 0.28
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.59
-
5'-AMP
P06961
-
0.29
-
tRNA
P06961
with ATP
1.08
-
tRNA
P06961
with 2'-AMP
1.68
-
tRNA
P06961
with 2',3'-cAMP
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.5
-
phosphate
P67095
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.025
-
-
pH 7.5, 30C, crude extract
1.49
-
P06961
+/- 0.08, ADP
2.36
-
P06961
+/- 0.10, 2',3'-cGMP
3.01
-
P06961
+/- 0.06, diphosphate
3.21
-
P06961
+/- 0.1, 2',3'-cAMP
3.71
-
P06961
+/- 0.17, 2'-CMP
4.03
-
P06961
+/- 0.20, CTP
4.53
-
P06961
+/- 0.14, ATP
5.8
-
P06961
+/- 0.47, CDP
12.4
-
P06961
+/- 0.34, p-nitrophenyl phosphate
12.93
-
-
-
17.9
-
P06961
+/- 0.6, NADP
100
-
-
about, pH 6, 37C, di(p-nitrophenyl) phosphate hydrolysis
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
6.5
-
hydrolysis of di-p-nitrophenyl phosphate
5.6
6.4
-
hydrolysis of di-p-nitrophenyl phosphate
6.4
-
-
3'-nucleotidase activity and cyclic phosphodiesterase activity against cyclic 2',3'-UMP
7
-
P06961
-
7.2
7.8
-
hydrolysis of 3'-AMP
7.4
8
-
hydrolysis of 2',3'-cyclic AMP, 2',3'-GMP, 2',3'-CMP and 2',3'-UMP
7.5
8.5
-
PdeB has slightly alkaline pH optimum (pH 7.5-8.5) for 3',5'-cAMP with 0.1 M Tris-HCl buffer
7.5
-
-
-
7.6
8.3
-
-
7.6
-
-
hydrolysis of 3'-UMP
7.8
-
-
hydrolysis of 3'-CMP
7.8
-
P53052, -
assay at
8.1
-
-
hydrolysis of 3'-GMP
8.2
-
-
hydrolysis of 3',5'-cyclic AMP
8.3
-
-
hydrolysis of 3'-AMP or di-p-nitrophenyl phosphate
8.4
-
-
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate
8.6
-
-
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
7.5
-
pH 5.0: about 40% of maximal activity, pH 9.0: 70-80% of maximal activity
6
9
-
about 55% of maximal activity at pH 6 and at pH 9
7.2
9
-
pH 7.2: about 45% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 3'-GMP
8
-
-
at pH-values greater than 8 activity declines sharply
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
37
-
P53052, -
assay at
37
-
-
assay at
40
45
-
-
45
-
-
hydrolysis of 2',3'-cyclic AMP
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
55
-
heat treatment at 50 or 55C for 5 min slightly activates the phosphodiesterase activity of PdeB against 3',5'-cAMP by about 1.2fold
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.1
-
-
chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Fusarium culmorum CECT 2148
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
P53052, -
enzyme is exported to the periplasmic space
-
Manually annotated by BRENDA team
additional information
-
results might indicate a surface localization
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34000
-
-
SDS-PAGE
65000
-
-
gel filtration
75000
-
-
gel filtration
79000
-
-
gel filtration
122000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
P53052, -
x * 71958, CpdB including the N-terminal signal sequence, calculated from the amino acid sequence, x * 68000, mature peptide without the signal sequence, SDS-PAGE
dimer
P06961
-
monomer
-
1 * 120000, SDS-PAGE
monomer
P06961
predominantly
monomer
-
1 * 70000, SDS-PAGE
monomer
Bacillus sp. A-40-2
-
1 * 120000, SDS-PAGE
-
oligomer
P06961
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
P53052, -
contains an N-terminal signal sequence of 25 amino acids
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
-
37C, loss of activity below
6
-
-
stable for at least 2 h at 37C
6
-
-
40C, 15 min, 80% loss of activity
7
11
-
40C, 15 min, fairly stable
12
-
-
40C, 15 min, 80% loss of activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
80
-
very stable to heat, medium concentrate of enzyme, 5 min, stable
50
-
-
denaturation above
55
-
-
partial inactivation
55
-
-
5 min, activates
55
-
-
partial inactivation
55
-
-
5 min, in absence of salts, 55% loss of activity, in presence of 0.2 mM MnCl2 13% loss of activity
55
-
-
inactivation
60
-
-
half-life: 12.2 min
65
-
-
partial inactivation
70
-
-
5 min, stable
70
-
-
5 min, loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stable
P06961
activity is not affected by several cycles of freezing and thawing
-
chymotrypsin, 10 min at 37C, 80% loss of phosphodiesterase activity and 3'-nucleotidase activity
-
trypsin, 10 min at 37C, 50% loss of phosphodiesterase activity
-
urea, 6.4 M, 53% loss of activity after 10 min, 20% loss of activity after 20 min
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-4C, pH 7.4-7.6, Tris buffer, 20-30% loss of activity after 2 months
-
4C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
P06961
-20C, several weeks, stable
-
4C or -12C, stable
-
-20C, pH 8.0, 20 mM Tris buffer, stable for at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial, activity towards 2',3'-cyclic nucleotides and 3',5'-cyclic nucleotides are associated with the same protein molecule
-
Ni-NTA agarose column chromatography
P67095
Ni-NTA agarose column chromatography
-
Talon affinity column chromatography
-
from the spent culture medium of phosphate-starved cells, 15fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli strain BL21(DE3)
P67095
genetic mapping of the cpdB locus
-
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli BL21 cells
-
cpdB gene, overexpression in Escherichia coli HB101, characterization of the gene
P53052, -
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
H98A
P67095
the mutation suppresses the 2',3'-cAMP phosphodiesterase activity of Rv0805 without adversely affecting hydrolysis of bis-p-nitrophenyl phosphate
H98D
P67095
the mutation suppresses the 2',3'-cAMP phosphodiesterase activity of Rv0805 without adversely affecting hydrolysis of bis-p-nitrophenyl phosphate
H98N
P67095
the mutation suppresses the 2',3'-cAMP phosphodiesterase specific activity to one-fifth the level of wild type Rv0805
H98A
-
the mutation suppresses the 2',3'-cAMP phosphodiesterase activity of Rv0805 without adversely affecting hydrolysis of bis-p-nitrophenyl phosphate
H98D
-
the mutation suppresses the 2',3'-cAMP phosphodiesterase activity of Rv0805 without adversely affecting hydrolysis of bis-p-nitrophenyl phosphate
H98N
-
the mutation suppresses the 2',3'-cAMP phosphodiesterase specific activity to one-fifth the level of wild type Rv0805
additional information
P53052, -
cpdB insertion mutant