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Literature summary for 3.1.4.16 extracted from

  • Ikura, Y.; Horikoshi, K.
    Manganese ion-dependent production of phosphodiesterase by alkalophilic Bacillus No. A-40-2 and its properties (1990), Agric. Biol. Chem., 54, 3205-3209.
No PubMed abstract available
Search for more literature for 3.1.4.16

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 0.1 mM, 59% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 bis(p-nitrophenyl)phosphate
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Bacillus sp. (in: Bacteria)
Ca2+ activates Bacillus sp. (in: Bacteria)
Mg2+ activates Bacillus sp. (in: Bacteria)
Mn2+ increases activity with bi-p-nitrophenyl phosphate, maximal activity at 10 mM Bacillus sp. (in: Bacteria)
Mo6+ activates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 1 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)
122000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) A-40-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.93
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Bacillus sp. (in: Bacteria) adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Bacillus sp. (in: Bacteria) A-40-2 adenosine + phosphate
-
 ?
3'-CMP + H2O
-
 Bacillus sp. (in: Bacteria) cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Bacillus sp. (in: Bacteria) A-40-2 cytidine + phosphate
-
 ?
3'-GMP + H2O
-
 Bacillus sp. (in: Bacteria) guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Bacillus sp. (in: Bacteria) A-40-2 guanosine + phosphate
-
 ?
3'-UMP + H2O
-
 Bacillus sp. (in: Bacteria) uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Bacillus sp. (in: Bacteria) A-40-2 uridine + phosphate
-
 ?
cyclic 2',3'-CMP + H2O
-
 Bacillus sp. (in: Bacteria) 3'-CMP
-
 ?
cyclic 2',3'-GMP + H2O
-
 Bacillus sp. (in: Bacteria) 3'-GMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Bacillus sp. (in: Bacteria) 3'-UMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Bacillus sp. (in: Bacteria) A-40-2 3'-UMP
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + p-nitrophenyl phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 40°C, 15 min, 80% loss of activity Bacillus sp. (in: Bacteria)
7 11 40°C, 15 min, fairly stable Bacillus sp. (in: Bacteria)
12
-
 40°C, 15 min, 80% loss of activity Bacillus sp. (in: Bacteria)